黄行许    正教授
所在学院生命科学与技术学院
研究方向遗传学、生殖生物学
联系方式huangxx@@shanghaitech.edu.cn
 
 个人简介 

1986 年毕业于江西农业大学牧医系,获学士学位;

1995 年毕业于华南农业大学动物医学系,获硕士学位;

1998 年毕业于南方医科大学基础医学院,获博士学位;

1998 年至 2000 年在中科院生物物理研究所从事博士后研究;

2001 年至 2007 年在美国贝勒医学院分子生理与生物物理系从事博士后研究;

2008 年至 2014 年受聘于南京大学模式动物研究所任教授、博导;

2015 年加盟上海科技大学生命科学与技术学院任副教授、研究员;

2018 年晋升为正教授。

 主要研究内容 

一直从事遗传学和生殖生物学相关工作。开发不同物种的同源重组(HR)、ZFN、TALEN、CRISPR、shRNA 等靶向技术:

1、通过解码和重编码揭示基因组和表观组与表型组的相关性,探讨配子形成和早期胚胎发育过程中的遗传和表观遗传调控规律;

2、构建各种基因工程细胞和动物疾病模型,进行临床细胞基因治疗试验研究。


迄今已在 Cell、Nature、NatureBiotechnology、Nature Methods 等杂志发表论文 100 余篇。基因编辑相关工作 2014 年被 Cell 评选为年度十大科学论文,被 Nature 评选为年度成功科学研究之一,被 MIT Technology Review 评选为年度十大突破性技术之一。Science、Nature做了点评,路透社、卫报、纽约时报等媒体报道;基因治疗相关工作在 2018 年第 22 届美国基因和细胞治疗年会上获评 Molecular Therapy年度最佳第一名。Science、Nature 做了点评,MIT Technology Review、Financial Times、NHK 等媒体专访。


 代表性论文 

1. Li, J.#, Liu, Z.#, Huang, S.#, Wang, X.,Li, G., Xu, Y., Yu, W., Chen, S., Zhang, Y., Ma, H., Ke, Z., Chen, J.*, Sun, Q.*, Huang, X.* (2019). Efficient baseediting in G/C-rich regions to model androgen insensitivity syndrome. CellRes. 29(2):174-176.

2. Shi, H.#, Zhang, X.#, Weng, Y.#, Lu, Z., Liu, Y., Lu, Z., Li,J., Hao, P., Zhang, Y., Zhang, F., Wu, Y., Delgado, J.Y., Su, Y., Patel, M.J.,Cao, X., Shen, B., Huang, X., Ming,G.L., Zhuang, X., Song, H.*, He, C.*, Zhou, T.* (2018). m6Afacilitates hippocampus-dependent learning and memory through YTHDF1.Nature 563(7730):249-253. (Zhou, T. is a co-PI of Huang’s group)

3. Zeng, Y.#,Li, J.#, Li, G.#, Huang, S., Yu, W., Zhang, Y., Chen, D.,Chen, J., Liu, J.*, Huang, X.*(2018). Correction of the Marfan Syndrome pathogenic FBN1 mutation by baseediting in human cells and heterozygous embryos. MolTher. 26(11):2631-2637. (Comment in:Science(https://www.sciencemag.org/news/2018/08/scientists-tweak-dna-viable-human-embryos); Nature 2019, 575: 553-555) (Selected by Molecular Therapy as the first of the 10 Best articles in 2018)

4. Wang, X.#,Li, J.#, Wang, Y.#, Yang, B.#, Wei, J.#,Wu, J., Wang, R., Huang, X.*,Chen, J.*, Yang, L.* (2018). Efficient base editing inmethylated regions with a human APOBEC3A-Cas9 fusion.Nat Biotechnol.36(10):946-949.

5. Jiang, W.#,Feng, S.#, Huang, S., Yu, W., Li, G., Yang, G., Liu, Y., Zhang, Y.,Zhang, L., Hou, Y., Chen, J., Chen, J.*, Huang, X.* (2018). Increasing the editing window and fidelity ofbase editing with scFv-APOBEC. Cell Res. 28(8):855-861.

6. Liu, Z.#, Lu, Z.#, Yang, G.#, Huang, S., Li, G.,Feng, S., Liu, Y., Li, J., Yu, W., Zhang, Y., Chen, J., Sun, Q.*, Huang, X.*(2018). Efficient generation of mouse models of human diseases via ABE- andBE-mediated base editing. Nature Commun. 9(1):2338. (Comment in: Nature 2019, 575: 553-555)

7. Li, X.#,Wang, Y.#, Liu, Y.#, Yang, B.#, Wang, X., Wei,J., Lu, Z., Zhang, Y., Wu, J., Huang, X.*,Yang, L.*, Chen, J.* (2018). Base editing with aCpf1-cytidine deaminase fusion. Nat Biotechnol.36(4): 324-327. (web of science highly cited paper).

8. Cui, Y.#,Niu, Y.#, Zhou, J.#, Chen, Y.#, Cheng, Y., Li,S., Ai, Z., Chu, C., Wang, H., Zheng, B., Chen, X., Sha, J., Guo, X. *,Huang, X. *, Ji, W.* (2018). Generation of a precise Oct4-hrGFP knockin cynomolgus monkeymodel via CRISPR/Cas9-assisted homologous recombination.CellRes. 28(3): 383-386.

9. Ke, Y. #,Xu, Y. #, Chen, X. #, Feng, S. #, Liu, Z.,Sun, Y., Yao, X., Li, F., Zhu, W., Gao, L., Chen, H., Du, Z., Xie, W., Xu, X., Huang, X.*, Liu, J.*(2017). 3D chromatin structures of mature gametes and structural reprogrammingduring mammalian embryogenesis. Cell 170(2): 367-381. (The 10Breakthroughs in biology of 2017 in China by BioArt).

10. Iyer, V.#,Shen, B.#, Zhang, W., Hodgkins, A., Keane, T., Huang, X.*, Skarnes, W.* (2015). Off-targetmutations are rare in Cas9-modified mice. Nat Methods 12(6): 479.

11. Niu, Y.#, Shen, B.#,Cui, Y.#, Chen, Y.#, Wang, J., Wang, L., Kang, Y., Zhao, X., Si, W., Li, W., Xiang, A.P., Zhou, J., Guo, X., Si, C., Hu, B., Dong, G.,Wang, H., Zhou, Z., Li, T., Tan, T., Pu, X., Wang, F., Ji, S., Zhou, Q., Huang, X.*, Ji, W.*, Sha, J.*(2014). Generation of gene-modified cynomolgus monkey via Cas9/RNA-mediatedgene targeting in one-cell embryos. Cell 156(4): 836-843. (Commentin: Science 2014, 343: 476-477; Nature 2014, 506: 8-8) (Selected byNatureas one of the events in the years in sciences: Wins and Losses) (Selected by Cellasone of the 10 Best articles in 2014) (web of science highly cited paper).

12. Wang, L.#,Zhang, J.#, Duan, J.#, Gao, X.#, Zhu, W.,Song, C., Yang, L., Zhang, J., Li, G., Ci, W., Li, W., Zhou, Q., Tang, F., He,C., Huang, X.*, Liu, J.*(2014). Programming and inheritance of parental DNA methylome in mammal. Cell157(4): 979-991. (Comment in: Cell Stem Sell2014, 15: 393-394).

13. Shen, B.#,Zhang, W.#, Zhang, J.#, Zhou, J., Wang, J., Chen, L.,Wang, L., Hogkins, A., Iyer, V.,Huang,X.*, Skarnes, W.* (2014). Efficient genomemodification in mice by CRISPR/Cas9 nickase without off-target effects. NatMethods 11(4): 399-402. (web of science highly cited paper)

14. Shen, B.#, Zhang, J.#, Wu, H.#, Wang, J., Ma, K., Li, Z., Zhang, X., Zhang, P., Huang,X.* (2013). Mouse gene knockout by Cas9/RNA-mediatedsite-specific gene targeting. Cell Res. 23(5): 720-723.

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