庄敏    助理教授、研究员
所在学院生命科学与技术学院
研究方向蛋白质泛素修饰
联系方式zhuangmin@@shanghaitech.edu.cn
 
 个人简介 
2003年本科毕业于南京大学基础学科教学强化部;2009年在美国田纳西大学健康医学中心获博士学位;2009年至2014年在加州大学旧金山分校进行博士后研究;2014年加入上海科技大学生命科学与技术学院任助理教授、研究员。
 主要研究内容 
随着近代癌症的分子生物学发病机理的研究和进步, 癌症的分子靶向治疗已经在临床实践中取得了显著的疗效。蛋白质的翻译后修饰,如蛋白质磷酸化和泛素化,已经成为了很多分子靶向药物调节的通路。泛素修饰过程中的各种酶也正成为新一代的分子靶向治疗的靶点。 我的研究方向将专注于在肿瘤中下调或有变异突变的泛素连接酶。我的目标是综合应用一系列的生化手段,研究蛋白质泛素修饰在肿瘤中的作用和识别新的靶向药物靶点。
 代表性论文 
1. Julien O.*, Zhuang M.*, Witta A.P., O’Donoghue A.J., Knudsen G.M., Craik C.S., Wells J.A.. Quantitative MS-based enzyme of caspases reveals distinct protein substrate specificities, hierarchies, and cellular roles. PNAS 113(14) E2001-E2010, 2016. (*equal contribution)

2. Li G., Ci W., Karmakar S., Chen K., Dhar R., Fan Z., Guo Z., Zhang J., Ke Y., Wang L., Zhuang M., Hu S., Li X., Zhou L., Li X., Calabrese M.F., Watson E.R., Prasad S.M., Rinker-Schaeffer C., Eggener S.E., Stricker T., Tian Y., Schulman B.A., Liu J., White K.P.. SPOP promotes tumorigenesis by acting as a key regulatory hub in kidney cancer. Cancer Cell 25, 1-14, 2014.
 
3. Zhuang M., Guan S., Wang H., Burlingame A.L., and Wells J.A.. Substrates of IAP ubiquitin ligases identified with a designed orthogonal E3 ligase, the NEDDylator. Mol Cell 49, 273-282, 2013. (Faculty of 1000: http://f1000.com/prime/717975954)
 
4. Crawford E.D., Seaman J.E., Agard N., Hsu G.W., Julien O., Mahrus S., Nguyen H., Shimbo K., Yoshihara H.A., Zhuang M., Chalkley R.J., Wells J.A.. The DegraBase: a database of proteolysis in healthy and apoptotic human cells. Mol Cell Proteomics 12, 813-824, 2013.
 
5. Zhuang M., Calabrese M.F., Liu J., Waddell M.B., Nourse A., Hammel M., Miller D.J., Walden H., Duda D.M., Seyedin S.N., Hoggard T., Harper J.W., White K.P., Schulman B.A.. Structures of SPOP-substrate complexes: insights into molecular architectures of BTB-Cul3 ubiquitin ligases. Mol Cell 36, 39-50, 2009. (Faculty of 1000: http://f1000.com/prime/1166991)
 
6. Jin Y., Zhuang M. & Hendershot L.M.. ERdj3, a luminal ER DnaJ homologue, binds directly to unfolded proteins in the mammalian ER: identification of critical residues. Biochemistry 48, 41-49, 2009.
 
7. Huang D.T., Zhuang M., Ayrault O., Schulman B.A.. Identification of conjugation specificity determinants unmasks vestigial preference for ubiquitin within the NEDD8 E2. Nat Struct Mol Biol 15, 280-287, 2008.
 
8. Huang D.T., Hunt H.W., Zhuang M., Ohi M.D., Holton J.M., Schulman B.A.. Basis for a ubiquitin-like protein thioester switch toggling E1-E2 affinity. Nature 445, 394-398, 2007. (Faculty of 1000: http://f1000.com/prime/1060912)
 
9. Huang D.T., Paydar A., Zhuang M., Waddell M.B., Holton J.M., Schulman B.A.. Structural basis for recruitment of Ubc12 by an E2 binding domain in NEDD8's E1. Mol Cell 17, 341-350, 2005.

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