庄敏    助理教授、研究员
所在学院生命科学与技术学院
研究方向蛋白质泛素修饰
联系方式zhuangmin@@shanghaitech.edu.cn


个人简介
2003年本科毕业于南京大学基础学科教学强化部;2009年在美国田纳西大学健康医学中心获博士学位;2009年至2014年在加州大学旧金山分校进行博士后研究;2014年加入上海科技大学生命科学与技术学院任助理教授、研究员。

主要研究内容
本课题组综合利用生物化学、细胞生物学、蛋白质组学和结构生物学等一系列的研究手段,开发应用研究蛋白质相互作用的新方法,  并将其应用到蛋白质泛素修饰领域, 研究泛素修饰在调节细胞器功能方面的作用。

代表性论文


1.Liu Q.*, Zheng J.*, Sun W., Huo Y., Zhang L., Hao P., Wang H.#, Zhuang  M#. A Proximity-tagging System to Identify Membrane Protein-Protein  Interactions. Nature Methods 15, 715-722, 2018. (Research Highlight:  Deane, C. PUP up the volume. Nat Chem Biol 14, 903, 2018)


2.Srinivasan S.R., Cesa L.C., Li X., Julien O., Zhuang M., Shao H.,  Chung J., Maillard I., Wells J.A., Duckett C., Gestwicki J.E. Heat shock protein  70 (Hsp70) suppresses RIP-1 dependent apoptotic and necroptotic cascades. Mol  Cancer Res 16(1):58–68, 2017.


3.Hill Z.B., Pollock S.B., Zhuang M., Wells J.A. Direct proximity  tagging of small molecule protein targets using an engineered NEDD8 ligase. J.  Am. Chem. Soc. 138(40): 13123-13126, 2016.


4. Julien O.*, Zhuang M.*, Witta A.P., O’Donoghue A.J.,  Knudsen G.M., Craik C.S., Wells J.A.. Quantitative MS-based enzyme of caspases  reveals distinct protein substrate specificities, hierarchies, and cellular  roles. PNAS 113(14) E2001-E2010, 2016. (*equal  contribution)

5. Li G., Ci W., Karmakar S., Chen K., Dhar R., Fan Z., Guo  Z., Zhang J., Ke Y., Wang L., Zhuang M., Hu S., Li X., Zhou L., Li X.,  Calabrese M.F., Watson E.R., Prasad S.M., Rinker-Schaeffer C., Eggener S.E.,  Stricker T., Tian Y., Schulman B.A., Liu J., White K.P.. SPOP promotes  tumorigenesis by acting as a key regulatory hub in kidney cancer. Cancer  Cell 25, 1-14, 2014.

6. Zhuang M., Guan S., Wang H.,  Burlingame A.L., and Wells J.A.. Substrates of IAP ubiquitin ligases identified  with a designed orthogonal E3 ligase, the NEDDylator. Mol Cell 49,  273-282, 2013. (Faculty of 1000: http://f1000.com/prime/717975954)

7.  Crawford E.D., Seaman J.E., Agard N., Hsu G.W., Julien O., Mahrus S., Nguyen H.,  Shimbo K., Yoshihara H.A., Zhuang M., Chalkley R.J., Wells J.A.. The  DegraBase: a database of proteolysis in healthy and apoptotic human cells.  Mol Cell Proteomics 12, 813-824, 2013.

8. Zhuang M.,  Calabrese M.F., Liu J., Waddell M.B., Nourse A., Hammel M., Miller D.J., Walden  H., Duda D.M., Seyedin S.N., Hoggard T., Harper J.W., White K.P., Schulman B.A..  Structures of SPOP-substrate complexes: insights into molecular architectures of  BTB-Cul3 ubiquitin ligases. Mol Cell 36, 39-50, 2009. (Faculty of 1000:  http://f1000.com/prime/1166991)

9. Jin Y., Zhuang M. &  Hendershot L.M.. ERdj3, a luminal ER DnaJ homologue, binds directly to unfolded  proteins in the mammalian ER: identification of critical residues.  Biochemistry 48, 41-49, 2009.

10. Huang D.T., Zhuang M.,  Ayrault O., Schulman B.A.. Identification of conjugation specificity  determinants unmasks vestigial preference for ubiquitin within the NEDD8 E2.  Nat Struct Mol Biol 15, 280-287, 2008.

11. Huang D.T., Hunt H.W.,  Zhuang M., Ohi M.D., Holton J.M., Schulman B.A.. Basis for a  ubiquitin-like protein thioester switch toggling E1-E2 affinity. Nature  445, 394-398, 2007. (Faculty of 1000:  http://f1000.com/prime/1060912)

12. Huang D.T., Paydar A., Zhuang  M., Waddell M.B., Holton J.M., Schulman B.A.. Structural basis for  recruitment of Ubc12 by an E2 binding domain in NEDD8's E1. Mol Cell 17,  341-350, 2005. 


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