Bo Sun    Assistant Professor, PI
InstituteSchool of Life Science and Technology
Research AreaSingle molecule biophysics

Dr. Sun received his bachelor degree from Jilin University in 2004, and obtained his Ph.D. degree in single molecule biophysics from Institute of Physics, Chinese Academy of Science in 2009. After graduation, he joined Cornell University and Howard Hughes Medical Institute and did his postdoc research from 2009 to 2015. Dr. Sun joined School of Life Science and Technology of ShanghaiTech University as an assistant professor, PI since September 2015.

Research Interests
Many biology reactions are too complex to fully comprehend through the use of conventional ensemble techniques. Studying one biological macromolecule at a time, or biology in singulo, can provide us with extraordinarily clear and often surprising views of these molecules in action. In the last 20 years, single molecule techniques, including magnetic tweezers and optical tweezers, have enabled significant advancement in the understanding of a wide variety of biomolecular systems, especially those involving DNA and associated proteins. My research interests are focused on understanding the mechanisms of molecular motors in the process of DNA replication, repair and transcription by using single‐molecule techniques and developing technological innovations to meet the challenges in the pursuits.

Selected Publications

1.    Sun, B.*, Singh, A., Sultana, S., Inman, J.T., Patel, S.S.,and Wang, M.D.* Helicase promotes replication re-initiating from an RNA transcript. Nat Commun, 9, 2306 (2018). (* Corresponding author)

2.    Sun, B. and Wang, M.D. Single-molecule optical-trapping techniques to study molecular mechanisms of a replisome. Methods Enzymol,582, 55-84 (2017).

3.    Sun, B. and Wang, M.D. Single-molecule perspectives on helicase mechanisms and functions. Crit Rev Biochem Mol Biol,51, 15-25 (2016).

4.    Sun, B., Pandey, M., Inman, J.T., Yang, Y., Kashlev, M., Patel, S.S.,and Wang, M.D. T7 replisome directly overcomes DNA damage. Nat Commun,6, 10260 (2015).

5.    Sun, B.#, Johnson, D.S.#, Patel, G., Smith, B.Y., Pandey, M., Patel, S.S.,and Wang, M.D. ATP-induced helicase slippage reveals highly coordinated subunits. Nature,478, 132-135 (2011). (# Equal Contribution)

6.    Patel, G., Johnson, D.S., Sun, B., Pandey, M., Yu, X., Egelman, E.H.,Wang, M.D., and Patel, S.S. A257T linker region mutant of T7 helicase-primase protein is defective in DNA loading and rescued by T7 DNA polymerase. J Biol Chem286, 20490-20499 (2011).

7.    Sun, B., Wei, K.J., Zhang, B., Zhang, X.H., Dou, S.X., Li, M., and Xi, X.G. Impediment of E. coli UvrD by DNA-destabilizing force reveals a strained-inchworm mechanism of DNA unwinding. EMBO J,27, 3279-3287 (2008).