Min Zhuang    Assistant Professor, PI
InstituteSchool of Life Science and Technology
Research AreaUbiquitination
Contact Info.zhuangmin@@shanghaitech.edu.cn

Dr. Min Zhuang graduated from the  Department for Intensive Instruction (current name: Kuang Yaming Honors School),  Nanjing University in 2003. She completed the graduate training at St Jude  Children’s Research Hospital and obtained her Ph.D. degree from The University  of Tennessee Health Science Center in 2009. She continued her postdoctoral  training in the Department of Pharmaceutical Chemistry at University of  California San Francisco. In 2014, she joined ShanghaiTech University as a  tenure-track assistant professor, PI in the School of Life Sciences and  Technology.

Research  Interests
We are using a multi-disciplinary approach  to study membrane-based ubiquitin ligases and their role in regulating organelle  function.

Selected  Publications

 1.Liu Q.*, Zheng J.*, Sun W., Huo Y., Zhang L., Hao P., Wang  H.#, Zhuang M#. A Proximity-tagging System to Identify Membrane  Protein-Protein Interactions. Nature Methods 15, 715-722, 2018. (Research  Highlight: Deane, C. PUP up the volume. Nat Chem Biol 14, 903, 2018)

2.Srinivasan S.R., Cesa L.C., Li X., Julien O., Zhuang M., Shao H.,  Chung J., Maillard I., Wells J.A., Duckett C., Gestwicki J.E. Heat shock protein  70 (Hsp70) suppresses RIP-1 dependent apoptotic and necroptotic cascades. Mol  Cancer Res 16(1):58–68, 2017.

3.Hill Z.B., Pollock S.B., Zhuang M., Wells J.A. Direct proximity  tagging of small molecule protein targets using an engineered NEDD8 ligase. J.  Am. Chem. Soc. 138(40): 13123-13126, 2016.

4. Julien O.*, Zhuang M.*, Witta A.P., O’Donoghue A.J.,  Knudsen G.M., Craik C.S., Wells J.A.. Quantitative MS-based enzyme of caspases  reveals distinct protein substrate specificities, hierarchies, and cellular  roles. PNAS 113(14) E2001-E2010, 2016. (*equal  contribution)

5. Li G., Ci W., Karmakar S., Chen K., Dhar R., Fan Z., Guo  Z., Zhang J., Ke Y., Wang L., Zhuang M., Hu S., Li X., Zhou L., Li X.,  Calabrese M.F., Watson E.R., Prasad S.M., Rinker-Schaeffer C., Eggener S.E.,  Stricker T., Tian Y., Schulman B.A., Liu J., White K.P.. SPOP promotes  tumorigenesis by acting as a key regulatory hub in kidney cancer. Cancer  Cell 25, 1-14, 2014.

6. Zhuang M., Guan S., Wang H.,  Burlingame A.L., and Wells J.A.. Substrates of IAP ubiquitin ligases identified  with a designed orthogonal E3 ligase, the NEDDylator. Mol Cell 49,  273-282, 2013. (Faculty of 1000: http://f1000.com/prime/717975954)

7.  Crawford E.D., Seaman J.E., Agard N., Hsu G.W., Julien O., Mahrus S., Nguyen H.,  Shimbo K., Yoshihara H.A., Zhuang M., Chalkley R.J., Wells J.A.. The  DegraBase: a database of proteolysis in healthy and apoptotic human cells.  Mol Cell Proteomics 12, 813-824, 2013.

8. Zhuang M.,  Calabrese M.F., Liu J., Waddell M.B., Nourse A., Hammel M., Miller D.J., Walden  H., Duda D.M., Seyedin S.N., Hoggard T., Harper J.W., White K.P., Schulman B.A..  Structures of SPOP-substrate complexes: insights into molecular architectures of  BTB-Cul3 ubiquitin ligases. Mol Cell 36, 39-50, 2009. (Faculty of 1000:  http://f1000.com/prime/1166991)

9. Jin Y., Zhuang M. &  Hendershot L.M.. ERdj3, a luminal ER DnaJ homologue, binds directly to unfolded  proteins in the mammalian ER: identification of critical residues.  Biochemistry 48, 41-49, 2009.

10. Huang D.T., Zhuang M.,  Ayrault O., Schulman B.A.. Identification of conjugation specificity  determinants unmasks vestigial preference for ubiquitin within the NEDD8 E2.  Nat Struct Mol Biol 15, 280-287, 2008.

11. Huang D.T., Hunt H.W.,  Zhuang M., Ohi M.D., Holton J.M., Schulman B.A.. Basis for a  ubiquitin-like protein thioester switch toggling E1-E2 affinity. Nature  445, 394-398, 2007. (Faculty of 1000:  http://f1000.com/prime/1060912)

12. Huang D.T., Paydar A., Zhuang  M., Waddell M.B., Holton J.M., Schulman B.A.. Structural basis for  recruitment of Ubc12 by an E2 binding domain in NEDD8's E1. Mol Cell 17,  341-350, 2005.