Xingxu Huang    Professor, PI
InstituteSchool of Life Science and Technology
Research AreaTranscriptional and epigenetic regulation of reprogramming and lineage commitment in germ cell development

Dr. Xingxu Huang graduated from Jiangxi Agriculture University in Nanchang in 1986, received a master degree from South China Agriculture University in Guangzhou in 1995 and a doctor degree from Southern Medical University in Guangzhou in 1998. He was a postdoctoral fellow in Institute of Biophysics, Chinese Academy of Sciences in Beijing from 1998-2000, and a postdoctoral research associate in Baylor College of Medicine in Houston, USA from 2001-2007. He was a professor and principal investigator in Model Animal Research Center, Nanjing University in Nanjing from 2008-2014. He joined the School of Life Science and Technology, ShanghaiTech University, as an Associate Professor, PI in 2015, and is promoted as a Tenured Full Professor in 2018.

Research Interests
The germ cell ensures the perpetuating and diversifying the genetic and epigenetic information across the generations. The germ cell development is accompanied by several fundamental biological processes such as reprogramming and lineage commitment which are regulated by transcriptional network and epigenetic modifications. After profiling the global transcriptome and epigenome, one of the next frontiers in biology is to site-specifically define and manipulate the transcriptional and epigenetic regulation of different biological processes. To this end, we have been developing genetic and epigenetic manipulation approaches, and applying such approaches combined with genome-wide analysis to understand the site-specific transcriptional and epigenetic regulation of germ cell development, including the elucidation and manipulation of the epigenetic and transcriptional network for reprogramming and lineage commitment.

Selected Publications
1. Iyer V#, Shen B#, Zhang W, Hodgkins A, Keane T, Huang X*, Skarnes W*. Off-target mutations are rare in Cas9-modified mice. Nat Methods 2015, 12(6): 479.

2. Chen Y#, Cui Y#, Shen B#, Niu Y, Zhao X, Wang L, Wang J, Li W, Zhou Q, Ji W*, Sha J*, Huang X*. Germline acquisition of Cas9/RNA-mediated gene modifications in monkeys. Cell Res. 2015, 25(2): 262-265.

3. Wang L, Zhang J, Duan J, Gao X, Zhu W, Song C, Yang L, Zhang J, Li G, Ci W, Li W, Zhou Q, Tang F, He C, Huang X*, Liu J*. Programming and inheritance of parental DNA methylome in mammal. Cell 2014, 157(4): 979-991.

4. Shen B, Zhang J, Zhang W, Zhou J, Wang J, Chen L, Wang L, Hogkins A, Iyer V, Huang X*, Skarnes W*. Efficient genome modification in mice by CRISPR/Cas9 nickase without off-target effects. Nature Methods 2014, 11(4): 399-402.

5. Niu Y, Shen B, Cui Y, Chen Y, Wang J, Wang L, Kang Y, Zhao X, Si W, Li W, Xiang AP, Zhou J, Guo X, Si C, Hu B, Dong G, Wang H, Zhou Z, Li T, Tan T, Pu X, Wang F, Ji S, Zhou Q, Huang X*, Ji W*, Sha J*. Generation of gene-modified cynomolgus monkey via Cas9/RNA-mediated gene targeting in one-cell embryos. Cell 2014, 156(4): 836-843. (Comment in: Science 2014, 343: 476-477. Nature 2014, 506: 8-8.) (This paper is on the recommended list at (Selected by Nature as one of the events in the years in sciences: Wins and Losses) (Selected by Cell as one of the 10 Best articles in 2014).