|Dr. Pengyu Huang received his BS degree from Huazhong University of Science and Technology in 2008, and was accepted into the Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences in the same year. During his Ph.D. study, he established a novel method to directly convert mouse fibroblasts to hepatocytes in vitro, which was selected as one of the China's Top 10 Scientific and Technological Advances of 2011. In 2013 he received his Ph.D. degree in cell biology. Then he joined the School of Life Science and Technology, ShanghaiTech University, as an assistant professor (tenure-track), PI in 2014. Currently he is focusing on the mechanism of liver regeneration regulation during chronic liver injuries.|
|1. Regulation of hepatocyte death and proliferation during liver diseases and liver regeneration|
Hepatocyte death induced by toxicants, hepatitis viruses and metabolic disorder could cause acute and chronic liver injury, and even liver failure. In most cases, recovery from liver injury largely dependents on compensatory hepatocyte proliferation. However, uncontrolled hepatocyte proliferation often leads to liver cancer. In our lab, we are focusing on the regulation of hepatocyte death, mechanisms of compensatory hepatocyte proliferation, and mechanism of hepatocellular carcinogenesis induced by chronic liver injuries.
Type I tyrosinemia mouse model for induced liver injury and hepatocyte transplantation
2. Biogenesis and modification of small RNAs in the liver
Small RNAs, such as miRNAs, piRNAs and tsRNAs, as well as their derivatives, regulate liver regeneration, liver metabolic diseases and liver cancer development. However, lack of study tools limits the study of small RNAs. Here, we are developing new methods to study the biogenesis, modification and functions of small RNAs in the liver.
1. Zhou, H., Liu, J., Zhou, C., Gao, N., Rao, Z., Li, H., Hu, X., Li, C., Yao, X., Shen, X., Sun, Y., Wei, Y., Liu, F., Ying, W., Zhang, J., Tang, C., Zhang, X., Xu, H., Shi, L., Cheng, L., Huang, P.*, Yang, H.* In vivo simultaneous transcriptional activation of multiple genes in the brain using CRISPR-dCas9-activator transgenic mice. Nature Neuroscience, online.
2. Yao, X., Wang, X., Liu, J., Shi, L., Huang, P.*, Yang, H.*, CRISPR/Cas9-mediated targeted integration in vivo using a homology-mediated end joining-based strategy. Journal of visualized experiments, online.
3. Wang, L., Zhou, Y., Dai, J., Jiang, S., Lu, Y., Sun, M., Gong, J.*, Huang, P.*, Yao, Y.* 2-Thiophene ethylamine modified hyaluronic acid with its application on hepatocytes culture, Materials Science and Engineering:C, 2018; 88.
1. Liu, J., Hu, X., Chen, J., Li, X., Wang, L., Wang, B., Peng, W., Yang, C., Li, Z., Chen, Y., Wang, Y., Li, C., Li, X., Yan, F., Wang, Y., Shang, C., Wang, X., Chen, T.*, Huang, P.* Pericentral hepatocytes produce insulin-like growth factor-2 to promote liver regeneration during selected injuries in mice. Hepatology, 2017; 66(6), 2002-2015. (Cited:1)
2. Yao, X., Wang, X., Liu, J., Hu, X., Shi, L., Shen, X., Ying, W., Sun, X., Wang, X., Huang, P.*, Yang, H.* CRISPR/Cas9-mediated precise targeted integration in vivo using a double cut donor with short homology arms. EBioMedicine. 2017; 20, 19-26. (Cited:6)
3. Yao, X., Wang, X., Hu, X., Liu, X., Liu, J., Zhou, H., Shen, X., Wei, Y., Huang, Z., Ying, W., Wang, Y., Nie, Y., Zhang, C., Li, S., Cheng, L., Wang, Q., Wu, Y., Huang, P., Sun, Q., Shi, L., Yang, H. Homology-mediated end joining-based targeted integration using CRISPR/Cas9. Cell Research, 2017; 27:801-814.
1. Huang, P., Li, B., Zheng, Y., Role of stem cells in the gastrointestinal tract and in the development of cancer. Stem Cells in Toxicology and Medicine, edited by Sahu SC, John Wiley & Sons, 2016 (book chapter)
2. Shi, X.#, Gao, Y.#, Yan, Y.#, Ma, H.#, Sun, L.#, Huang, P.#, Ni, X., Zhang, L., Zhao, X., Ren, H., Hu, D., Zhou, Y., Tian, F., Ji, Y., Cheng, X., Pan, G., Ding, Y., Hui, L., Improved survival of porcine acute liver failure by a bioartificial liver device implanted with induced human functional hepatocytes. Cell Research, 2016; 26:206-216. (Cited:30)
3. Pu, W., Zhang, H., Huang, X., Tian, X., He, L., Wang, Y., Zhang, L., Liu, Q., Li, Y., Li, Y., Zhao, H., Liu, K., Lu, J., Zhou, Y., Huang, P., Nie, Y., Yan, Y., Hui, L., Lui, K., Zhou, B. Mfsd2a+ hepatocytes repopulate the liver during injury and regeneration. Nature Communications, 2016; 7, 13369.
1. Huang, P.#, Zhang, L.#, Gao, Y.#, He, Z.#, Yao, D., Wu, Z., Cen, J., Chen, X., Liu, C., Hu, Y., Lai, D., Hu, Z., Chen, L., Zhang, Y., Ma, X., Pan, G., Wang, X., Hui, L. Direct reprogramming of human fibroblasts to functional and expandable hepatocytes. Cell Stem Cell, 2014; 14(3):370–84. (Cited:196)
# co-first author; * corresponding author