Hanhui Ma    Assistant Professor, PI
InstituteSchool of Life Science and Technology
Research AreaGenome Editing, Epigenetics and Cell Fate Decision
Contact Info.mahh@@shanghaitech.edu.cn

Dr. Ma received his BS degree from Beijing Technology and Business University in 1997 and his Ph.D. degree from Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences in 2004. Dr. Ma received his postdoc training at University of Massachusetts Medical School from 2004-2011 and then he was promoted to be research specialist. Dr. Ma joined ShanghaiTech University in June,2018 as a tenure-track assistant professor, PI in School of Life Sciences and Technology.

Research Interests
The On-Off switch of genes is critical for cell fate decision, development and human disease. My group will combine single cell genomics and cutting-edge microscopy technology to identify what genes are turned on or off in cell type-specific or disease-specific manners. How are these genes precisely controlled in space and time? We will use CRISPR-based live cell DNA and RNA tracing system to understand the spatiotemporal regulation of genes during the physiological process, and we will harness CRISPR-based editing technology to reverse aberrant gene expression in the disease models.

Selected Publications

(# Co-first author, * Corresponding author)

1. Ma H*, Tu LC, Naseri A, Chung YC, Grunwald D, Zhang S, Pederson T. (2018) CRISPR-Sirius: RNA scaffolds for signal amplification in genome imaging. Nat. Methods. 15: 928-931.
2. Ma H*, Reyes-Gutierrez P, Pederson T. (2018) A CRISPR-Based Selective Gene Inhibition Method Reveals Dynamic Features of a Cell Nucleus Nanobody Related to the Disease Myotonic Dystrophy. Small Methods. 1700400.  
3. Chuai G, Ma H, Yan J, Chen M, Hong N, Xue D, Zhou C, Zhu C, Chen K, Duan B, Gu F, Qu S, Huang D, Wei J, Liu Q. (2018) DeepCRISPR: Optimized CRISPR Guide RNA Design by Deep Learning. Genome Biol. 19(1): 80.  
4. Ma H*, Tu LC, Naseri A, Chung YC, Grunwald D, Zhang S, Pederson T. (2017) CRISPR-Based Imaging Reveals Cell-Cycle-Dependent Chromosome Dynamics in Living Cells. BioRxiv 195966; doi.org/10.1101/195966 
5. Ma H*, Tu LC, Naseri A, Huisman M, Zhang S, Grunwald D, Pederson T. (2016) Multiplexed Labeling of Genomic Loci with dCas9 and Engineered sgRNAs using CRISPRainbow. Nat. Biotechnol34: 528-530.  
6. Ma H#, Tu LC#, Naseri A, Huisman M, Zhang S, Grunwald D, Pederson T*. (2016) CRISPR-Cas9 Nuclear Dynamics and Target Recognition in Living Cells. J. Cell Biol. 214:529-537.  (Highlighted in an “In Focus” editorial) 
7. Ma H*, Naseri A, Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T*. (2015) Multicolor CRISPR Labeling of Chromosomal Loci in Human Cells. Proc. Natl. Acad. Sci. USA 112: 3002-3007.  
8. Ma H, McLean JR, Gould KL, McCollum D. (2014) An Efficient Fluorescent Protein-Based Multifunctional Affinity Purification Approach in Mammalian Cells. Methods Mol. Biol. 1177:175-191. (Invited protocol) 
9. Ma H*, Reyes-Gutierrez P, Pederson T*. (2013) Visualization of Repetitive DNA Sequences in Human Chromosomes with Transcription Activator-Like Effectors. Proc. Natl. Acad. Sci. USA. 110: 21048-21053. 
10. Ma, H* & Pederson T*. (2013) The Nucleolus Stress Response is Coupled to an ATR-Chk1-Mediated G2 Arrest.  Mol. Biol. Cell 24: 1334-1342.  
11. Ma H, McLean JR, Chao LF, Mana-Capelli S, Paramasivam M, Hagstrom KA, Gould KL, McCollum D. (2012) A Highly Efficient Multifunctional Tandem Affinity Purification Approach Applicable to Diverse Organisms. Mol. Cell. Proteomics11: 501-511.  
12. Ma H & Pederson T. (2008) Nucleophosmin is a Binding Partner of Nucleostemin in Human Osteosarcoma Cells.  Mol. Biol. Cell 19: 2870-2875. 
13. Ma H* & Pederson T. (2008) Nucleostemin: a Multiplex Regulator of Cell-Cycle Progression. Trends Cell Biol. 18: 575-579. 
14. Ma H & Pederson T. (2007) Depletion of the Nucleolar Protein Nucleostemin Causes G1 Cell Cycle Arrest via the p53 PathwayMol. Biol. Cell 18: 2630-2635. 
15. Ma HH, Yang L, Yang XY, Xu ZP, Li BL. (2003) Bacterial Expression, Purification, and in vitro N-Myristoylation of Fusion Hepatitis B Virus preS1 with the Native-Type N-terminus. Protein Expr. Purif. 27: 49-54.