陈佳    助理教授、研究员
所在学院生命科学与技术学院
研究方向DNA修复、基因编辑与癌症发生
联系方式chenjia@@shanghaitech.edu.cn


个人简介
2002年本科毕业于南开大学生命科学学院生物化学系;2009年在中科院上海生物化学与细胞生物学研究所获博士学位;2009年至2014年在美国国立卫生研究院糖尿病、消化道和肾脏疾病研究所(NIH/NIDDK)进行博士后研究工作。2014年11月加入上海科技大学生命科学与技术学院任助理教授、研究员。2016年5月获得上海市科委浦江人才计划资助。

主要研究内容
物种繁衍与基因组的稳定性密切相关,这在细胞内是由一系列精巧而复杂的DNA修复机制负责维持的。然而在某些生理、病理状态下细胞会激活错误倾向性DNA修复机制。因此DNA修复精确性和错误倾向性之间的平衡对于疾病发生、衰老和进化都具有重要的意义。
我们实验室将探索由DNA修复引发的突变在基因编辑、疾病发生以及衰老等过程中所起的作用, 主要集中于三个方面: 1)研究DNA修复在CRISPR/Cas介导基因编辑过程中引发突变的分子机制;2)在机制研究基础上开发新型基因编辑技术;3)鉴定并分析参与DNA修复引发突变的新分子和新通路;4)探索DNA修复引发突变在癌症发生以及衰老过程中的作用。我们的研究将有助于拓展基因编辑系统,揭示癌症发生的新机制以及完善衰老的DNA损伤学说。

代表性论文

(# co-first author, * corresponding author)

1.     Xiaosa Li#, Ying Wang#, Yajing Liu#, Bei Yang#, Xiao Wang, Jia Wei, Zongyang Lu, Yuxi Zhang, Jing Wu, Xingxu Huang*, Li Yang* and Jia Chen*. Base editing with a cpf1-cytidine deaminase fusion. Nat Biotechnol, 2018, 36: 324-327

2.     Liqun Lei#, Hongquan Chen#, Wei Xue#, Bei Yang#, Bian Hu#, Jia Wei, Lijie Wang, Yiqiang Cui, Wei Li, Jianying Wang, Lei Yan, Wanjing Shang, Jimin Gao, Jiahao Sha, Min Zhuang, Xingxu Huang, Bin Shen*, Li Yang* and Jia Chen*. APOBEC3 induces mutations during repair of CRISPR–Cas9-generated DNA breaks. Nat Struct Mol Biol, 2018, 25: 45-52

3.     Lijie Wang#, Wei Xue#, Lei Yan#, Xiaosa Li, Jia Wei, Miaomiao Chen, Jing Wu, Bei Yang*, Li Yang* and Jia Chen*. Enhanced base editing by co-expression of free uracil DNA glycosylase inhibitor. Cell Res, 2017, 27: 1289-1292

4.     Guanglei Li, Yajing Liu, Yanting Zeng, Jianan Li, 
Lijie Wang, Guang Yang, Dunjin Chen, Xiaoyun Shang, Jia Chen, Xingxu Huang* and Jianqiao Liu*. Highly efficient and precise base editing in discarded human tripronuclear embryos. Protein Cell, 2017, 8: 776-779

5.     Bei Yang*, Xiaosa Li, Liqun Lei and Jia Chen*. APOBEC: from mutator to editor. J Genet Genomics, 2017, 44: 423-437

6.     Jia Chen and Anthony V. Furano*. Breaking bad: The mutagenic effect of DNA repair. DNA Repair, 2015, 32: 43-51

7.     Jia Chen, Brendan F. Miller and Anthony V. Furano*. Repair of naturally occurring mismatches can induce mutations in flanking DNA. eLife, 2014, 3: e02001

·      Featured in Insight Article by Samuel H. Wilson. The dark side of DNA repair. eLife, 2014, 3: e03068

8.     Guang-Jing Hu#, Jia Chen#, Xiao-Nan Zhao#, Jia-Jia Xu, Dong-Qing Guo, Ming Lu, Ming Zhu, Ying Xiong, Qin Li, Catherine CY Chang, Bao-Liang Song, Ta-Yuan Chang and Bo-Liang Li*. Production of ACAT1 56-kDa isoform in human cells via trans-splicing involving the ampicillin resistance gene. Cell Res, 2013, 23: 1007-1024 (Cover story)

·      Featured in Research Highlight Article by Christian Preußer and Albrecht Bindereif. Exo-endo trans splicing: a new way to link. Cell Res, 2013, 23: 1071-1072

9.     Lei Lei, Ying Xiong, Jia Chen, Jin-Bo Yang, Yi Wang, Xin-Ying Yang, Cantherine C. Y. Chang, Bao-Liang Song, Ta-Yuan Chang and Bo-Liang Li*. TNF-alpha stimulates the ACAT1 expression in differentiating monocytes to promote the CE-laden cell formation. J Lipid Res, 2009, 50: 1057-1067

10.   Xiao-Nan Zhao#, Jia Chen#, Lei Lei, Guang-Jing Hu, Ying Xiong, Jia-Jia Xu, Qin Li, Xin-Ying Yang, Catherine CY Chang, Bao-Liang Song, Ta-Yuan Chang and Bo-Liang Li*. The optional long 5'-untranslated region of human ACAT1 mRNAs impairs the production of ACAT1 protein by promoting its mRNA decay. Acta Biochim Biophys Sin, 2009, 41: 30-41

11.   Jia Chen#, Xiao-Nan Zhao#, Li Yang, Guang-Jing Hu, Ming Lu, Ying Xiong, Xin-Ying Yang, Catherine CY Chang, Bao-Liang Song, Ta-Yuan Chang and Bo-Liang Li*. RNA secondary structures located in the interchromosomal region of human ACAT1 chimeric mRNA are required to produce the 56-kDa isoform. Cell Res, 2008, 18: 921-936

12.   Bo-Liang Li*, Ta-Yuan Chang, Jia Chen, Catherine CY Chang and Xiao-Nan Zhao. Human ACAT1 gene expression and its involvement in the development of atherosclerosis. Future Cardiol, 2006, 2: 93-99

13.   Li Yang, Oneil Lee, Jia Chen, Jiang Chen, Catherine C.Y. Chang, Pei Zhou, Zhen-Zhen Wang, Han-Hui Ma, Hui-Fang Sha, Jiu-Xian Feng, Yi Wang, Xin-Ying Yang, Li Wang, Ruhong Dong, Kim Ornvold, Bo-Liang Li* and Ta-Yuan Chang*. Human acyl-coenzyme A:cholesterol acyltransferase 1(Acat1) sequences located in two different chromosomes (7 and 1) are required to produce a novel ACAT1 isoenzyme with additional sequence at the N-terminal. J Biol Chem, 2004, 279: 46253-46262

14.   Li Yang, Jin-Bo Yang, Jia Chen, Guang-Yao Yu, Pei Zhou, Lei Lei, Zhen-Zhen Wang, Catherine C.Y. Chang, Xin-Ying Yang, Ta-Yuan Chang* and Bo-Liang Li*. Enhancement of human ACAT1 gene expression to promote the macrophage-derived foam cell formation by dexamethasone. Cell Res, 2004, 14: 315-323 (Cover story)

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