陈佳    助理教授、研究员
所在学院生命科学与技术学院
研究方向DNA修复引发突变及癌症发生的分子机制
联系方式chenjia@@shanghaitech.edu.cn


个人简介
2002年本科毕业于南开大学生命科学学院生物化学系;2009年在中科院上海生命科学研究院生物化学与细胞生物学研究所获博士学位;2009年至2014年在美国国立卫生研究院(NIH)进行博士后研究工作。2014年11月加入上海科技大学生命科学与技术学院任助理教授、研究员。2016年5月获得上海市科委浦江人才计划资助。

主要研究内容
物种繁衍依赖于基因组的稳定性,这在细胞内是由一系列精巧而复杂的DNA修复机制负责维持的。然而在某些生理、病理状态下细胞会激活错误倾向性DNA修复机制。因此DNA修复的精确性和错误倾向性之间的平衡对于包括癌症在内的疾病发生、衰老和进化都有着重要的意义。我们实验室将探索由DNA修复引发的突变在基因编辑、疾病发生、衰老以及表观遗传学变化等过程中所起的作用, 主要集中于三个方面: 1)研究DNA修复在CRISPR/Cas9介导基因编辑过程中引发突变的分子机制,并改进现有基因编辑技术;2)鉴定并分析参与DNA修复引发突变的新分子和新通路;3)探索DNA修复引发突变在癌症发生过程中的作用。我们的研究将有助于提高基因编辑技术的精确性,揭示癌症治疗的新靶标以及完善衰老的DNA损伤学说。

代表性论文
1.Lijie Wang#, Wei Xue#, Lei Yan#, Xiaosa Li, Jia Wei, Miaomiao Chen, Jing Wu, Bei Yang*, Li Yang* and Jia Chen*. Enhanced base editing by co-expression of free uracil DNA glycosylase inhibitor.Cell Res, 2017 Aug 29, doi: doi:10.1038/cr.2017.111 

2.Guanglei Li, Yajing Liu, Yanting Zeng, Jianan Li, 
Lijie Wang, Guang Yang, Dunjin Chen, Xiaoyun Shang, Jia Chen, Xingxu Huang* and Jianqiao Liu*. Highly efficient and precise base editing in discarded human tripronuclear embryos. Protein Cell, 2017 Aug 19, doi: 10.1007/s13238-017-0458-7

3.Bei Yang*, Xiaosa Li, Liqun Lei and Jia Chen*. APOBEC: from mutator to editor. J Genet Genomics, 2017 Aug 7, doi: 10.1016/j.jgg.2017.04.009

4. Jia Chen and Anthony V. Furano*. Breaking bad: The mutagenic effect of DNA repair. DNA Repair, 2015, 32: 43-51

5. Jia Chen, Brendan F. Miller and Anthony V. Furano*. Repair of naturally occurring mismatches can induce mutations in flanking DNA. eLife, 2014, 3: e02001
•Featured in Insight Article by Samuel H. Wilson. The dark side of DNA repair. eLife, 2014, 3: e03068

6. Guang-Jing Hu#, Jia Chen#, Xiao-Nan Zhao#, Jia-Jia Xu, Dong-Qing Guo, Ming Lu, Ming Zhu, Ying Xiong, Qin Li, Catherine CY Chang, Bao-Liang Song, Ta-Yuan Chang and Bo-Liang Li*. Production of ACAT1 56-kDa isoform in human cells via trans-splicing involving the ampicillin resistance gene. Cell Res, 2013, 23: 1007-1024 (Cover story)
•Featured in Research Highlight Article by Christian Preußer and Albrecht Bindereif. Exo-endo trans splicing: a new way to link. Cell Res, 2013, 23: 1071-1072

7. Lei Lei, Ying Xiong, Jia Chen, Jin-Bo Yang, Yi Wang, Xin-Ying Yang, Cantherine C. Y. Chang, Bao-Liang Song, Ta-Yuan Chang and Bo-Liang Li*. TNF-alpha stimulates the ACAT1 expression in differentiating monocytes to promote the CE-laden cell formation. J Lipid Res, 2009, 50: 1057-1067

8. Xiao-Nan Zhao#, Jia Chen#, Lei Lei, Guang-Jing Hu, Ying Xiong, Jia-Jia Xu, Qin Li, Xin-Ying Yang, Catherine CY Chang, Bao-Liang Song, Ta-Yuan Chang and Bo-Liang Li*. The optional long 5'-untranslated region of human ACAT1 mRNAs impairs the production of ACAT1 protein by promoting its mRNA decay. Acta Biochim Biophys Sin, 2009, 41: 30-41

9. Jia Chen#, Xiao-Nan Zhao#, Li Yang, Guang-Jing Hu, Ming Lu, Ying Xiong, Xin-Ying Yang, Catherine CY Chang, Bao-Liang Song, Ta-Yuan Chang and Bo-Liang Li*. RNA secondary structures located in the interchromosomal region of human ACAT1 chimeric mRNA are required to produce the 56-kDa isoform. Cell Res, 2008, 18: 921-936

10. Bo-Liang Li*, Ta-Yuan Chang, Jia Chen, Catherine CY Chang and Xiao-Nan Zhao. Human ACAT1 gene expression and its involvement in the development of atherosclerosis. Future Cardiol, 2006, 2: 93-99


11. Li Yang, Oneil Lee, Jia Chen, Jiang Chen, Catherine C.Y. Chang, Pei Zhou, Zhen-Zhen Wang, Han-Hui Ma, Hui-Fang Sha, Jiu-Xian Feng, Yi Wang, Xin-Ying Yang, Li Wang, Ruhong Dong, Kim Ornvold, Bo-Liang Li* and Ta-Yuan Chang*. Human acyl-coenzyme A:cholesterol acyltransferase 1(Acat1) sequences located in two different chromosomes (7 and 1) are required to produce a novel ACAT1 isoenzyme with additional sequence at the N-terminal. J Biol Chem, 2004, 279: 46253-46262


12. Li Yang, Jin-Bo Yang, Jia Chen, Guang-Yao Yu, Pei Zhou, Lei Lei, Zhen-Zhen Wang, Catherine C.Y. Chang, Xin-Ying Yang, Ta-Yuan Chang* and Bo-Liang Li*. Enhancement of human ACAT1 gene expression to promote the macrophage-derived foam cell formation by dexamethasone. Cell Res, 2004, 14: 315-323 (Cover story)

(# co-first author, * corresponding author)

浏览次数:16135

返回原图
/