2016年科研成果

12月

1.       Efficient generation of B2m-null pigs via injection of zygote with TALENs.

Wang Y, Du Y, Zhou X, Wang L, Li J, Wang F, Huang Z, Huang XX, Wei H. Sci Rep. 2016 Dec 16;6:38854. doi: 10.1038/srep38854.

11月

2.       CRISPR-Cas9-mediated disruption of PD-1 on human T cells for adoptive cellular therapies of EBV positive gastric cancer.

Su S, Zou Z, Chen F, Ding N, Du J, Shao J, Li L, Fu Y, Hu B, Yang Y, Sha H, Meng F, Wei J, Huang XX, Liu B. Oncoimmunology. 2016 Nov 22;6(1):e1249558. doi: 10.1080/2162402X.2016.1249558. eCollection 2017.

3.       Mfsd2a+ hepatocytes repopulate the liver during injury and regeneration.

Pu W, Zhang H, Huang X, Tian X, He L, Wang Y, Zhang L, Liu Q, Li Y, Li Y, Zhao H, Liu K, Lu J, Zhou Y, Huang P, Nie Y, Yan Y, Hui L, Lui KO, Zhou B. Nat Commun. 2016 Nov 18;7:13369. doi: 10.1038/ncomms13369.

4.       Structural basis for DNA recognition by STAT6.

Li J , Rodriguez JP , Niu F, Pu M, Wang J, Hung LW, Shao Q, Zhu Y, Ding W, Liu Y, Da Y, Yao Z, Yang J6, Zhao Y, Wei GH, Cheng G, Liu ZJ, Ouyang S. Proc Natl Acad Sci U S A. 2016 Nov 15;113(46):13015-13020. Epub 2016 Nov 1.

5.       Melatonin Suppresses Toll Like Receptor 4-Dependent Caspase-3 Signaling Activation Coupled with Reduced Production of Proinflammatory Mediators in Hypoxic Microglia.

Yao L, Lu P, Ling EA. PLoS One. 2016 Nov 3;11(11):e0166010. doi: 10.1371/journal.pone.0166010. eCollection 2016.

10月

6.       Crystal Structure of the Human Cannabinoid Receptor CB1.

Hua T, Vemuri K, Pu M, Qu L, Han GW, Wu Y, Zhao S, Shui W, Li S, Korde A, Laprairie RB, Stahl EL, Ho JH, Zvonok N, Zhou H, Kufareva I, Wu B, Zhao Q, Hanson MA, Bohn LM, Makriyannis A, Stevens RC, Liu ZJ.  Cell. 2016 Oct 20;167(3):750-762.e14. doi: 10.1016/j.cell.2016.10.004.

7.       Disruption of FGF5 in Cashmere Goats Using CRISPR/Cas9 Results in More Secondary Hair Follicles and Longer Fibers.

Wang X, Cai B, Zhou J, Zhu H, Niu Y, Ma B, Yu H, Lei A, Yan H, Shen Q, Shi L, Zhao X, Hua J, Huang X, Qu L, Chen Y. PLoS One. 2016 Oct 18;11(10):e0164640. doi: 10.1371/journal.pone.0164640. eCollection 2016.

8.       Direct Proximity Tagging of Small Molecule Protein Targets Using an Engineered NEDD8 Ligase.

Hill ZB, Pollock SB, Zhuang M, Wells JA. J Am Chem Soc. 2016 Oct 12;138(40):13123-13126. Epub 2016 Sep 28.

9.       Whole-Brain Monosynaptic Afferent Inputs to Basal Forebrain Cholinergic System.

Hu R, Jin S, He X, Xu F, Hu J. Front Neuroanat. 2016 Oct 10;10:98. eCollection 2016.

10.       Porcine Zygote Injection with Cas9/sgRNA Results in DMD-Modified Pig with Muscle Dystrophy.

Yu HH, Zhao H, Qing YB, Pan WR, Jia BY, Zhao HY, Huang XX, Wei HJ.  Int J Mol Sci. 2016 Oct 9;17(10). pii: E1668.

11.   The Cytoophidium and Its Kind: Filamentation and Compartmentation of Metabolic Enzymes.

Liu JL. Annu Rev Cell Dev Biol. 2016 Oct 6;32:349-372. Epub 2016 Jun 27.

12.   Antigen Receptor Nanoclusters: Small Units with Big Functions.

Liu W, Wang H, Xu C. Trends Immunol. 2016 Oct;37(10):680-689. doi: 10.1016/j.it.2016.07.007. Epub 2016 Aug 20.

9月

13.   SPRY1 regulates mammary epithelial morphogenesis by modulating EGFR-dependent stromal paracrine signaling and ECM remodeling.

Koledova Z, Zhang X, Streuli C, Clarke RB, Klein OD, Werb Z, Lu P.Proc Natl Acad Sci U S A. 2016 Sep 27;113(39):E5731-40. doi: 10.1073/pnas.1611532113. Epub 2016 Sep 12.

14.   Native phasing of x-ray free-electron laser data for a G protein-coupled receptor.

Batyuk A, Galli L, Ishchenko A, Han GW, Gati C, Popov PA, Lee MY, Stauch B, White TA, Barty A, Aquila A, Hunter MS, Liang M, Boutet S, Pu M, Liu ZJ, Nelson G, James D, Li C, Zhao Y, Spence JC, Liu W, Fromme P, Katritch V, Weierstall U, Stevens RC, Cherezov V.  Sci Adv. 2016 Sep 23;2(9):e1600292. eCollection 2016 Sep.

15.   aKMT Catalyzes Extensive Protein Lysine Methylation in the Hyperthermophilic Archaeon Sulfolobus islandicus but is Dispensable for the Growth of the Organism.

Chu Y, Zhu Y, Chen Y, Li W, Zhang Z, Liu D, Wang T, Ma J, Deng H, Liu ZJ, Ouyang S, Huang L.Mol Cell Proteomics. 2016 Sep;15(9):2908-23. doi: 10.1074/mcp.M115.057778. Epub 2016 Jun 21.

16.    Mitrocomin from the jellyfish Mitrocoma cellularia with deleted C-terminal tyrosine reveals a higher bioluminescence activity compared to wild type photoprotein.

Burakova LP, Natashin PV, Markova SV, Eremeeva EV, Malikova NP, Cheng C, Liu ZJ, Vysotski ES.J Photochem Photobiol B. 2016 Sep;162:286-297. doi: 10.1016/j.jphotobiol.2016.06.054. Epub 2016 Jul 2.

17.   TALEN-based generation of a cynomolgus monkey disease model for human microcephaly.

Ke Q, Li W, Lai X, Chen H, Huang L, Kang Z, Li K, Ren J, Lin X, Zheng H, Huang W, Ma Y, Xu D, Chen Z, Song X, Lin X, Zhuang M, Wang T, Zhuang F, Xi J, Mao FF, Xia H, Lahn BT, Zhou Q, Yang S, Xiang AP. Cell Res. 2016 Sep;26(9):1048-61. doi: 10.1038/cr.2016.93. Epub 2016 Aug 9.

18.   STRIPAK complexes in cell signaling and cancer.

Shi Z, Jiao S, Zhou Z. Oncogene. 2016 Sep 1;35(35):4549-57. doi: 10.1038/onc.2016.9. Epub 2016 Feb 15.

8月

19.   Lunapark Is a Component of a Ubiquitin Ligase Complex Localized to the Endoplasmic Reticulum Three-way Junctions.

Zhao Y, Zhang T, Huo H, Ye Y, Liu Y.J Biol Chem. 2016 Aug 26;291(35):18252-62. doi: 10.1074/jbc.M116.737783. Epub 2016 Jul 7.

20.   Multiplex gene editing via CRISPR/Cas9 exhibits desirable muscle hypertrophy without detectable off-target effects in sheep.

Wang X, Niu Y, Zhou J, Yu H, Kou Q, Lei A, Zhao X, Yan H, Cai B, Shen Q, Zhou S, Zhu H, Zhou G, Niu W, Hua J, Jiang Y, Huang XX, Ma B, Chen Y. Sci Rep. 2016 Aug 26;6:32271. doi: 10.1038/srep32271.

21.   Coupling of TRPV6 and TMEM16A in epithelial principal cells of the rat epididymis.

Gao da Y, Zhang BL, Leung MC, Au SC, Wong PY, Shum WW. J Gen Physiol. 2016 Aug;148(2):161-82. doi: 10.1085/jgp.201611626.

7月

22.   Novel Chemical Ligands to Ebola Virus and Marburg Virus Nucleoproteins Identified by Combining Affinity Mass Spectrometry and Metabolomics Approaches.

Fu X, Wang Z, Li L, Dong S, Li Z, Jiang Z, Wang Y, Shui W.Sci Rep. 2016 Jul 12;6:29680. doi: 10.1038/srep29680.

23.   Increasing the efficiency of CRISPR/Cas9-mediated precise genome editing in rats by inhibiting NHEJ and using Cas9 protein.

Ma Y, Chen W, Zhang X, Yu L, Dong W, Pan S, Gao S, Huang XX, Zhang L. RNA Biol. 2016 Jul 2;13(7):605-12. doi: 10.1080/15476286.2016.1185591. Epub 2016 May 10.

24.   Structural and Biochemical Insight into the Mechanism of Rv2837c from Mycobacterium tuberculosis as a c-di-NMP Phosphodiesterase.

He Q, Wang F, Liu S, Zhu D, Cong H, Gao F, Li B, Wang H, Lin Z, Liao J, Gu L. J Biol Chem. 2016 Jul 1;291(27):14386-7. doi: 10.1074/jbc.A115.699801.

6月

25.   Major Variations in HIV-1 Capsid Assembly Morphologies Involve Minor Variations in Molecular Structures of Structurally Ordered Protein Segments.

 Lu JX, Bayro MJ, Tycko R. J Biol Chem. 2016 Jun 17;291(25):13098-112. doi: 10.1074/jbc.M116.720557. Epub 2016 Apr 19.

26.   Somatic human ZBTB7A zinc finger mutations promote cancer progression.

Liu XS, Liu Z, Gerarduzzi C, Choi DE, Ganapathy S, Pandolfi PP, Yuan ZM. Oncogene. 2016 Jun 9;35(23):3071-8. doi: 10.1038/onc.2015.371. Epub 2015 Oct 12.

27.   Electrophysiological characterization of the archaeal transporter NCX_Mj using solid supported membrane technology.

Barthmes M, Liao J, Jiang Y, Brüggemann A, Wahl-Schott C. J Gen Physiol. 2016 Jun;147(6):485-96. doi: 10.1085/jgp.201611587.

28.   Mechanism of extracellular ion exchange and binding-site occlusion in a sodium/calcium exchanger.

Liao J, Marinelli F#, Lee C, Huang Y, Faraldo-Gómez JD, Jiang Y. Nat Struct Mol Biol. 2016 Jun;23(6):590-599. doi: 10.1038/nsmb.3230. Epub 2016 May 16.

29.   A gain-of-function mutation in Msl10 triggers cell death and wound-induced hyperaccumulation of jasmonic acid in Arabidopsis.

Zou Y, Chintamanani S, He P, Fukushige H, Yu L, Shao M, Zhu L, Hildebrand DF, Tang X, Zhou JM. J Integr Plant Biol. 2016 Jun;58(6):600-9. doi: 10.1111/jipb.12427. Epub 2015 Nov 13.

5月

30.   An activation-induced IL-15 isoform is a natural antagonist for IL-15 function.

Zhao L, Hu B, Zhang Y, Song Y, Lin D, Liu Y, Mei Y, Sandikin D, Sun W, Zhuang M, Liu H. Sci Rep. 2016 May 11;6:25822. doi: 10.1038/srep25822.

31.  Current advances in the functional studies of fatty acids and fatty acid-derived lipids in C. elegans.

Ying L, Zhu H. Worm. 2016 May 4;5(3):e1184814. eCollection 2016.

32.   MLKL forms cation channels.

Xia B, Fang S, Chen X, Hu H, Chen P, Wang H, Gao Z. Cell Res. 2016 May;26(5):517-28. doi: 10.1038/cr.2016.26. Epub 2016 Apr 1.

33.  In vitro expression and analysis of the 826 human G protein-coupled receptors.

Lv X, Liu J, Shi Q, Tan Q, Wu D, Skinner JJ, Walker AL, Zhao L, Gu X, Chen N, Xue L, Si P, Zhang L, Wang Z, Katritch V, Liu ZJ, Stevens RC. Protein Cell. 2016 May;7(5):325-37. doi: 10.1007/s13238-016-0263-8. Epub 2016 Apr 16.

4月

34.    Generation of an Oocyte-Specific Cas9 Transgenic Mouse for Genome Editing.

Zhang L, Zhou J, Han J, Hu B, Hou N, Shi Y, Huang XX, Lou X. PLoS One. 2016 Apr 27;11(4):e0154364. doi: 10.1371/journal.pone.0154364. eCollection 2016.

35.   Quantitative MS-based enzymology of caspases reveals distinct protein substrate specificities, hierarchies, and cellular roles.

Julien O, Zhuang M, Wiita AP, O'Donoghue AJ, Knudsen GM, Craik CS, Wells JA. Proc Natl Acad Sci U S A. 2016 Apr 5;113(14):E2001-10. doi: 10.1073/pnas.1524900113. Epub 2016 Mar 22.

3月

36.   Crystal structure of hGEF-H1 PH domain provides insight into incapability in phosphoinositide binding.

Jiang Y, Jiang H, Zhou S, Meng B, Liu ZJ, Ouyang S. Biochem Biophys Res Commun. 2016 Mar 18;471(4):621-7. doi: 10.1016/j.bbrc.2016.01.150. Epub 2016 Jan 26.

37.   Precise regulation of the guidance receptor DMA-1 by KPC-1/Furin instructs dendritic branching decisions.

Dong X, Chiu H, Park YJ, Zou W, Zou Y, Özkan E, Chang C, Shen K. Elife. 2016 Mar 14;5. pii: e11008. doi: 10.7554/eLife.11008.

38.   H3K4 Methyltransferase Set1a Is A Key Oct4 Coactivator Essential for Generation of Oct4 Positive Inner Cell Mass.

Fang L, Zhang J, Zhang H, Yang X, Jin X, Zhang L, Skalnik DG, Jin Y, Zhang Y, Huang XX, Li J, Wong J. Stem Cells. 2016 Mar;34(3):565-80. doi: 10.1002/stem.2250. Epub 2016 Jan 19.

2月

39.   New insights into the structural basis of DNA recognition by HINa and HINb domains of IFI16.

Ni X, Ru H, Ma F, Zhao L, Shaw N, Feng Y, Ding W, Gong W, Wang Q, Ouyang S, Cheng G, Liu ZJ. J Mol Cell Biol. 2016 Feb;8(1):51-61. doi: 10.1093/jmcb/mjv053. Epub 2015 Aug 5.

40.   Improved survival of porcine acute liver failure by a bioartificial liver device implanted with induced human functional hepatocytes.

Shi XL, Gao Y, Yan Y, Ma H, Sun L, Huang P, Ni X, Zhang L, Zhao X, Ren H, Hu D, Zhou Y, Tian F, Ji Y, Cheng X, Pan G, Ding YT, Hui L2. Cell Res. 2016 Feb;26(2):206-16. doi: 10.1038/cr.2016.6. Epub 2016 Jan 15.

1月

41.    CRISPR-Cas9 mediated efficient PD-1 disruption on human primary T cells from cancer patients.

Su S, Hu B, Shao J, Shen B, Du J, Du Y, Zhou J, Yu L, Zhang L, Chen F, Sha H, Cheng L, Meng F, Zou Z, Huang XX, Liu B. Sci Rep. 2016 Jan 28;6:20070. doi: 10.1038/srep20070.

42.   Systematic identification of genes with a cancer-testis expression pattern in 19 cancer types.

Wang C, Gu Y, Zhang K, Xie K, Zhu M, Dai N, Jiang Y, Guo X, Liu M, Dai J, Wu L, Jin G, Ma H, Jiang T, Yin R, Xia Y, Liu L, Wang S, Shen B, Huo R, Wang Q, Xu L, Yang L, Huang XX, Shen H, Sha J, Hu Z. Nat Commun. 2016 Jan 27;7:10499. doi: 10.1038/ncomms10499.

43.    All Ca2+-binding loops of light-sensitive ctenophore photoprotein berovin bind magnesium ions: The spatial structure of Mg2+-loaded apo-berovin.

Burakova LP,Natashin PV,Malikova NP,Niu FF,Pu MC,Vysotski ES ,Liu ZJ. J Photochem Photobiol B. 2016 Jan;154:57-66. doi: 10.1016/j.jphotobiol.2015.11.012

44.    Efficient Generation of Gene-Modified Pigs Harboring Precise Orthologous Human Mutation via CRISPR/Cas9-Induced Homology-Directed Repair in Zygotes.

Zhou X, Wang L, Du Y, Xie F, Li L, Liu Y, Liu C, Wang S, Zhang S, Huang XX, Wang Y, Wei H. Hum Mutat. 2016 Jan;37(1):110-8. doi: 10.1002/humu.22913. Epub 2015 Oct 23.

45.   A CRISPR-Based Toolbox for Studying T Cell Signal Transduction.

Chi S, Weiss A, Wang H.Biomed Res Int. 2016;2016:5052369. doi: 10.1155/2016/5052369. Epub 2016 Feb 1.

46.   Single-molecule perspectives on helicase mechanisms and functions.

Sun B, Wang MD. Crit Rev Biochem Mol Biol. 2016;51(1):15-25. doi: 10.3109/10409238.2015.1102195. Epub 2015 Nov 5.

47.   Role of Stem Cells in the Gastrointestinal Tract and in the Development of Cancer.

Huang P, Li B, Zheng YW (2016). Role of Stem Cells in the Gastrointestinal Tract and in the Development of Cancer. In Stem Cells in Toxicology and Medicine (John Wiley & Sons, Ltd), pp. 363-374.