2017年科研成果

12月

1.       CTP synthase forms the cytoophidium in human hepatocellular carcinoma.

Chang CC, Jeng YM, Peng M, Keppeke GD, Sung LY, Liu JL.  Exp Cell Res. 2017 Dec 15;361(2):292-299. doi: 10.1016/j.yexcr.2017.10.030. Epub 2017 Oct 31.

2.       Overexpression of Human-Derived DNMT3A Induced Intergenerational Inheritance of Active DNA Methylation Changes in Rat Sperm.

Zheng X, Li Z, Wang G, Li Z, Liang A, Wang H, Dai Y, Huang XX, Chen X, Ma Y, Sun F. Front Genet. 2017 Dec 12;8:207. doi: 10.3389/fgene.2017.00207. eCollection 2017.

3.       Dynamic regulation of CD28 conformation and signaling by charged lipids and ions.

Yang W, Pan W, Chen S, Trendel N, Jiang S, Xiao F, Xue M, Wu W, Peng Z, Li X, Ji H, Liu X, Jiang H, Wang H, Shen H, Dushek O, Li H, Xu C. Nat Struct Mol Biol. 2017 Dec;24(12):1081-1092. doi: 10.1038/nsmb.3489. Epub 2017 Oct 23.

4.     Pericentral hepatocytes produce insulin-like growth factor-2 to promote liver regeneration during selected injuries in mice.

Liu J, Hu X, Chen J, Li X, Wang L, Wang B, Peng W, Yang C, Li Z, Chen Y, Wang YJ, Li C, Li X, Yan F, Wang Y, Shang C, Wang X, Chen T, Huang P. Hepatology. 2017 Dec;66(6):2002-2015. doi: 10.1002/hep.29340. Epub 2017 Nov 6.

11月

5.       Structural insights into the committed step of bacterial phospholipid biosynthesis.

Li Z, Tang Y, Wu Y, Zhao S, Bao J, Luo Y, Li D. Nat Commun. 2017 Nov 22;8(1):1691. doi: 10.1038/s41467-017-01821-9.

10月

6.       Ligand identification of the adenosine A(2A) receptor in self-assembled nanodiscs by affinity mass spectrometry

Ma J , Lu Y , Wu D, Peng Y, Loa-Kum-Cheung W ,Peng C, Quinn RJ, Shui WQ, Liu ZJ .  ANALYTICAL METHODS. 2017 Oct 28;9(40): 5851-5858. doi: 10.1039/c7ay01891f.

7.       Requirement for CCNB1 in mouse spermatogenesis.

Tang JX, Li J, Cheng JM, Hu B, Sun TC, Li XY, Batool A, Wang ZP, Wang XX, Deng SL, Zhang Y, Chen SR, Huang XX, Liu YX. Cell Death Dis. 2017 Oct 26;8(10):e3142. doi: 10.1038/cddis.2017.555.

8.       Wisp2 disruption represses Cxcr4 expression and inhibits BMSCs homing to injured liver.

Qin D#, Yan Y#, Hu B#, Zhang W, Li H, Li X, Liu S, Dai D, Hu X, Huang XX, Zhang L. Oncotarget. 2017 Oct 24;8(58):98823-98836. doi: 10.18632/oncotarget.22006. eCollection 2017 Nov 17.

9.       YTHDC1 mediates nuclear export of N6-methyladenosine methylated mRNAs.

Roundtree IA, Luo GZ, Zhang Z, Wang X, Zhou T, Cui Y, Sha J, Huang XX, Guerrero L, Xie P, He E, Shen B, He C. Elife. 2017 Oct 6;6. pii: e31311. doi: 10.7554/eLife.31311.

10.   Electrospinning of PVA/sericin nanofiber and the effect on epithelial-mesenchymal transition of A549 cells.

Yan S, Li X, Dai J, Wang Y, Wang B, Lu Y, Shi J, Huang P, Gong J, Yao Y. Mater Sci Eng C Mater Biol Appl. 2017 Oct 1;79:436-444. doi: 10.1016/j.msec.2017.05.048. Epub 2017 May 10.

11.   Suppression of protein tyrosine phosphatase N23 predisposes to breast tumorigenesis via activation of FYN kinase.

Zhang S, Fan G, Hao Y, Hammell M, Wilkinson JE, Tonks NK. Genes Dev. 2017 Oct 1;31(19):1939-1957. doi: 10.1101/gad.304261.117. Epub 2017 Oct 24.

12.   Highly efficient and precise base editing in discarded human tripronuclear embryos.

Li G, Liu Y, Zeng Y, Li J, Wang L, Yang G, Chen D, Shang X, Chen J, Huang XX, Liu J. Protein Cell. 2017 Oct;8(10):776-779. doi: 10.1007/s13238-017-0458-7.

13.   Enhanced base editing by co-expression of free uracil DNA glycosylase inhibitor.

Wang L, Xue W, Yan L, Li X, Wei J, Chen M, Wu J, Yang B, Yang L, Chen J. Cell Res. 2017 Oct;27(10):1289-1292. doi: 10.1038/cr.2017.111. Epub 2017 Aug 29.

9月

14.   Design and Characterization of a Human Monoclonal Antibody that Modulates Mutant Connexin 26 Hemichannels Implicated in Deafness and Skin Disorders.

Xu L, Carrer A, Zonta F, Qu Z, Ma P, Li S, Ceriani F, Buratto D, Crispino G, Zorzi V, Ziraldo G, Bruno F, Nardin C, Peres C, Mazzarda F, Salvatore AM, Raspa M, Scavizzi F, Chu Y, Xie S, Yang X, Liao J, Liu X, Wang W, Wang S, Yang G, Lerner RA, Mammano F, Front Mol Neurosci. 2017 Sep 22;10:298. doi: 10.3389/fnmol.2017.00298. eCollection 2017.

15.   Sparks of the CRISPR explosion: Applications in medicine and agriculture.

Liu JL. J Genet Genomics. 2017 Sep 20;44(9):413-414. doi: 10.1016/j.jgg.2017.09.006.

16.   APOBEC: From mutator to editor.

Yang B, Li X, Lei L, Chen J. J Genet Genomics. 2017 Sep 20;44(9):423-437. doi: 10.1016/j.jgg.2017.04.009. Epub 2017 Aug 7.

17.   Key Elements for Designing and Performing a CRISPR-Cas9 based Genetic Screen.

Shang W, Wang F, Fan G, Wang H. J Genet Genomics. 2017 Sep 20;44(9):439-449. doi: 10.1016/j.jgg.2017.09.005. Epub 2017 Sep 22.

18.   Fibroblasts in an endocardial fibroelastosis disease model mainly originate from mesenchymal derivatives of epicardium.

Zhang H, Huang X, Liu K, Tang J, He L, Pu W, Liu Q, Li Y, Tian X, Wang Y, Zhang L, Yu Y, Wang H, Hu R, Wang F, Chen T, Wang QD, Qiao Z, Zhang L, Lui KO, Zhou B. Cell Res. 2017 Sep;27(9):1157-1177. doi: 10.1038/cr.2017.103. Epub 2017 Aug 15.

8月

19.   PPR polyadenylation factor defines mitochondrial mRNA identity and stability in trypanosomes.

Zhang L, Sement FM, Suematsu T, Yu T, Monti S, Huang L, Aphasizhev R1, Aphasizheva I. EMBO J. 2017 Aug 15;36(16):2435-2454. doi: 10.15252/embj.201796808. Epub 2017 Jul 6.

20.   Making human cardiomyocytes up to date: Derivation, maturation state and perspectives.

Kolanowski TJ, Antos CL, Guan K. Int J Cardiol. 2017 Aug 15;241:379-386. doi: 10.1016/j.ijcard.2017.03.099. Epub 2017 Mar 24.

21.   Whole-cell Patch-clamp Recordings of Isolated Primary Epithelial Cells from the Epididymis.

Zhang BL, Gao DY, Zhang XX, Shi S, Shum W. J Vis Exp. 2017 Aug 3;(126). doi: 10.3791/55700.

7月

22.   Crystal structures of agonist-bound human cannabinoid receptor CB1.

Hua T, Vemuri K, Nikas SP, Laprairie RB, Wu Y, Qu L, Pu M, Korde A, Jiang S, Ho JH, Han GW, Ding K, Li X, Liu H, Hanson MA, Zhao S, Bohn LM, Makriyannis A, Stevens RC, Liu ZJ. Nature. 2017 Jul 27;547(7664):468-471. doi: 10.1038/nature23272. Epub 2017 Jul 5.

23.   DNA-binding mechanism of the Hippo pathway transcription factor TEAD4.

Shi Z, He F, Chen M, Hua L, Wang W, Jiao S, Zhou Z. Oncogene. 2017 Jul 27;36(30):4362-4369. doi: 10.1038/onc.2017.24. Epub 2017 Apr 3.

24.   Diverse Supramolecular Nanofiber Networks Assembled by Functional Low-Complexity Domains.

An B, Wang X, Cui M, Gui X, Mao X, Liu Y, Li K, Chu C, Pu J, Ren S, Wang Y, Zhong G, Lu TK, Liu C, Zhong C. ACS Nano. 2017 Jul 25;11(7):6985-6995. doi: 10.1021/acsnano.7b02298. Epub 2017 Jun 19.

25.   Identification of a hybrid myocardial zone in the mammalian heart after birth.

Tian X, Li Y, He L, Zhang H, Huang X, Liu Q, Pu W, Zhang L, Li Y, Zhao H, Wang Z, Zhu J, Nie Y, Hu S, Sedmera D, Zhong TP, Yu Y, Zhang L, Yan Y, Qiao Z, Wang QD, Wu SM, Pu WT1, Anderson RH, Zhou B. Nat Commun. 2017 Jul 20;8(1):87. doi: 10.1038/s41467-017-00118-1.

26.   Ionic CD3-Lck interaction regulates the initiation of T-cell receptor signaling.

Li L, Guo X, Shi X, Li C, Wu W, Yan C, Wang H, Li H, Xu C. Proc Natl Acad Sci U S A. 2017 Jul 18;114(29):E5891-E5899. doi: 10.1073/pnas.1701990114. Epub 2017 Jun 28.

27.   3D Chromatin Structures of Mature Gametes and Structural Reprogramming during Mammalian Embryogenesis.

Ke Y, Xu Y, Chen X, Feng S, Liu Z, Sun Y, Yao X, Li F, Zhu W, Gao L, Chen H, Du Z, Xie W, Xu X, Huang XX, Liu J. Cell. 2017 Jul 13;170(2):367-381.e20. doi: 10.1016/j.cell.2017.06.029.

28.   Loss of Sun2 promotes the progression of prostate cancer by regulating fatty acid oxidation.

Yajun C#, Chen Y#, Xiaosa L#, Xiao W, Jia C, Zhong W, Bin X. Oncotarget. 2017 Jul 12;8(52):89620-89630. doi: 10.18632/oncotarget.19210. eCollection 2017 Oct 27.

6月

29.   Gene knockout of Zmym3 in mice arrests spermatogenesis at meiotic metaphase with defects in spindle assembly checkpoint.

Hu X, Shen B, Liao S, Ning Y, Ma L, Chen J, Lin X, Zhang D, Li Z, Zheng C, Feng Y, Huang XX, Han C. Cell Death Dis. 2017 Jun 29;8(6):e2910. doi: 10.1038/cddis.2017.228.

30.   A Novel Lid-Covering Peptide Inhibitor of Nicotinic Acetylcholine Receptors Derived from alphaD-Conotoxin GeXXA.

Yang L, Tae HS, Fan Z, Shao X, Xu S, Zhao S, Adams DJ, Wang C. Mar Drugs. 2017 Jun 5;15(6). pii: E164. doi: 10.3390/md15060164.

31.   Response Patterns of GABAergic Neurons in the Anterior Piriform Cortex of Awake Mice.

Hu R, Zhang J, Luo M, Hu J. Cereb Cortex. 2017 Jun 1;27(6):3110-3124. doi: 10.1093/cercor/bhw175.

32.   CRISPR/Cas9 - Mediated Precise Targeted Integration In Vivo Using a Double Cut Donor with Short Homology Arms.

Yao X, Wang X, Liu J, Hu X, Shi L, Shen X, Ying W, Sun X, Wang X, Huang P, Yang H. EBioMedicine. 2017 Jun;20:19-26. doi: 10.1016/j.ebiom.2017.05.015. Epub 2017 May 11.

33.   Human GLP-1 receptor transmembrane domain structure in complex with allosteric modulators.

Song G, Yang D, Wang Y, de Graaf C, Zhou Q, Jiang S, Liu K, Cai X, Dai A, Lin G, Liu D, Wu F, Wu Y, Zhao S, Ye L, Han GW, Lau J, Wu B, Hanson MA, Liu ZJ, Wang MW, Stevens RC. Nature. 2017 Jun 8;546(7657):312-315. doi: 10.1038/nature22378. Epub 2017 May 17.

34.   Homology-mediated end joining-based targeted integration using CRISPR/Cas9.

Yao X, Wang X, Hu X, Liu Z, Liu J, Zhou H, Shen X, Wei Y, Huang Z, Ying W, Wang Y, Nie YH, Zhang CC, Li S, Cheng L, Wang Q, Wu Y, Huang P, Sun Q, Shi L, Yang H. Cell Res. 2017 Jun;27(6):801-814. doi: 10.1038/cr.2017.76. Epub 2017 May 19.

35.   NEDD4 controls spermatogonial stem cell homeostasis and stress response by regulating messenger ribonucleoprotein complexes.

Zhou Z, Kawabe H, Suzuki A, Shinmyozu K, Saga Y. Nat Commun. 2017 Jun 6;8:15662. doi: 10.1038/ncomms15662.

36.   Structural Basis for Apelin Control of the Human Apelin Receptor.

Ma Y, Yue Y, Ma Y, Zhang Q, Zhou Q, Song Y, Shen Y, Li X, Ma X, Li C, Hanson MA, Han GW, Sickmier EA, Swaminath G, Zhao S, Stevens RC, Hu LA, Zhong W, Zhang M, Xu F. Structure. 2017 Jun 6;25(6):858-866.e4. doi: 10.1016/j.str.2017.04.008. Epub 2017 May 18.

5月

37.   Crystal structure of a multi-domain human smoothened receptor in complex with a super stabilizing ligand.

Zhang X, Zhao F, Wu Y, Yang J, Han GW, Zhao S, Ishchenko A, Ye L, Lin X, Ding K, Dharmarajan V, Griffin PR, Gati C, Nelson G, Hunter MS, Hanson MA, Cherezov V, Stevens RC, Tan W, Tao H, Xu F.Nat Commun. 2017 May 17;8:15383. doi: 10.1038/ncomms15383.

38.   Critical roles of CTP synthase N-terminal in cytoophidium assembly.

Huang Y, Wang JJ, Ghosh S, Liu JL. Exp Cell Res. 2017 May 15;354(2):122-133. doi: 10.1016/j.yexcr.2017.03.042. Epub 2017 Mar 22.

4月

39.   A structurally guided dissection-then-evolution strategy for ligand optimization of smoothened receptor.

Ye L, Ding K, Zhao F, Liu X, Wu Y, Liu Y, Xue D, Zhou F, Zhang X, Stevens RC, Xu F, Zhao S, Tao H. Medchemcomm. 2017 Apr 28;8(6):1332-1336. doi: 10.1039/c7md00104e. eCollection 2017 Jun 1.

3月

40.   A Convenient Cas9-based Conditional Knockout Strategy for Simultaneously Targeting Multiple Genes in Mouse.

Chen J, Du Y, He X, Huang XX, Shi YS. Sci Rep. 2017 Mar 31;7(1):517. doi: 10.1038/s41598-017-00654-2.

41.   Quantitative proteomics reveal proteins enriched in tubular endoplasmic reticulum of Saccharomyces cerevisiae.

Wang X, Li S, Wang H, Shui W, Hu J. Elife. 2017 Mar 13;6. pii: e23816. doi: 10.7554/eLife.23816.

2月

42.   A hypothalamic circuit that controls body temperature.

Zhao ZD, Yang WZ, Gao C, Fu X, Zhang W, Zhou Q, Chen W, Ni X, Lin JK, Yang J, Xu XH, Shen WL. Proc Natl Acad Sci U S A. 2017 Feb 21;114(8):2042-2047. doi: 10.1073/pnas.1616255114. Epub 2017 Jan 4.

43.   Optimization of Acquisition and Data-Processing Parameters for Improved Proteomic Quantification by Sequential Window Acquisition of All Theoretical Fragment Ion Mass Spectrometry.

Li S, Cao Q, Xiao W, Guo Y, Yang Y, Duan X, Shui W. J Proteome Res. 2017 Feb 3;16(2):738-747. doi: 10.1021/acs.jproteome.6b00767. Epub 2017 Jan 3.

44.   The emerging roles of the DDX41 protein in immunity and diseases.

Jiang Y, Zhu Y, Liu ZJ, Ouyang S. Protein Cell. 2017 Feb;8(2):83-89. doi: 10.1007/s13238-016-0303-4. Epub 2016 Aug 9.

1月

45.   Exploring the Mutational Robustness of Nucleic Acids by Searching Genotype Neighborhoods in Sequence Space.

Zhou Q, Sun X, Xia X, Fan Z, Luo Z, Zhao S, Shakhnovich E, Liang H. J Phys Chem Lett. 2017 Jan 19;8(2):407-414. doi: 10.1021/acs.jpclett.6b02769. Epub 2017 Jan 5.

46.   Structural variation in amyloid-beta fibrils from Alzheimer's disease clinical subtypes.

Qiang W, Yau WM, Lu JX, Collinge J, Tycko R. Nature. 2017 Jan 12;541(7636):217-221. doi: 10.1038/nature20814. Epub 2017 Jan 4.

47.   GGPP-Mediated Protein Geranylgeranylation in Oocyte Is Essential for the Establishment of Oocyte-Granulosa Cell Communication and Primary-Secondary Follicle Transition in Mouse Ovary.

Jiang C, Diao F, Sang YJ, Xu N, Zhu RL, Wang XX, Chen Z, Tao WW, Yao B, Sun HX, Huang XX, Xue B, Li CJ. PLoS Genet. 2017 Jan 10;13(1):e1006535. doi: 10.1371/journal.pgen.1006535. eCollection 2017 Jan.

48.   Biochemical features of the adhesion G protein-coupled receptor CD97 related to its auto-proteolysis and HeLa cell attachment activities.

Yang LY, Liu XF, Yang Y, Yang LL, Liu KW, Tang YB, Zhang M, Tan MJ, Cheng SM, Xu YC, Yang HY, Liu ZJ, Song GJ, Huang W. Acta Pharmacol Sin. 2017 Jan;38(1):56-68. doi: 10.1038/aps.2016.89. Epub 2016 Sep 19.

49.   Single-Molecule Optical-Trapping Techniques to Study Molecular Mechanisms of a Replisome.

Sun B, Wang MD. Methods Enzymol. 2017;582:55-84. doi: 10.1016/bs.mie.2016.08.001. Epub 2016 Oct 27.

50.   A 3D Fibroblast-Epithelium Co-culture Model for Understanding Microenvironmental Role in Branching Morphogenesis of the Mammary Gland.

Koledova Z, Lu P. Methods Mol Biol. 2017;1501:217-231.

51.   Integrating MS1 and MS2 Scans in High-Resolution Parallel Reaction Monitoring Assays for Targeted Metabolite Quantification and Dynamic 13C-Labeling Metabolism Analysis.

Li Z, Li Y, Chen W, Cao Q, Guo Y, Wan N, Jiang X, Tang YJ, Wang Q, Shui W. Anal Chem. 2017 Jan 3;89(1):877-885. doi: 10.1021/acs.analchem.6b03947. Epub 2016 Dec 14.

52.   Solid-State Structure of Abeta (Aβ) in Alzheimer's Disease.

Lu JX, Dong XQ, Zhang JJ. Protein Pept Lett.2017;24(4):322-330. doi: 10.2174/0929866524666170208155108.

53.   Building Cre Knockin Rat Lines Using CRISPR/Cas9.

Ma Y, Zhang L, Huang XX.  Methods in Molecular Biology 2017;1642, 37-52.

54.   TRPA1 mediates sensation of the rate of temperature change in Drosophila larvae.

Luo J, Shen WL, Montell C. Nat Neurosci. 2017 Jan;20(1):34-41. doi: 10.1038/nn.4416. Epub 2016 Oct 17.

55.   Chapter 15 - Mouse Models of Epigenetic Inheritance: Classification, Mechanisms, and Experimental Strategies A2 - Tollefsbol, Trygve O.

Mao S, Li Y, Liu B, Chi T.  In Handbook of Epigenetics (Second edition) (Academic Press), pp. 231-243.

56.   Genomic Tagging of AGO1 using CRISPR/Cas9-mediated Homologous Recombination.

        Ghosh S, Liu JL.  Methods Mole Biol.1680:217-235.