中文信息

合成生物学研究组

课题组长

姓名：

Hisashi Tadakuma助理教授研究员博士生导师, PhD, 助理教授

职务：

所在院所：

生命科学与技术学院

荣誉称号：

教育经历：

1991/04—1995/03，日本早稻田大学，学士
1995/04—1997/03，日本早稻田大学，硕士
1997/04—2001/03，日本早稻田大学，博士课程
2004/03—2004/03，日本早稻田大学，博士

博士后及工作经历：

2001—2003，Waseda University, Takashi Funatsu lab，Assistant Professor
2003—2008，University of Tokyo, Michio Tomishige lab，Assistant Professor
2008—2010，University of Tokyo, Takuya Ueda / Hideki Taguchi lab，Postdoc
2010—2014/12，University of Tokyo, Takuya Ueda lab，Assistant Professor
2015/01—2016，Kyoto University, Yoshie Harada lab，Postdoct
2016—2019，Osaka University, Yoshie Harada lab，Assistant Professor
2019/11—至今，上海科技大学，生命科学与技术学院，助理教授 (TENURE-TRACK)

课题组简介

研究方向：

以合成生物学和生物物理学为基础的纳米生物学

研究内容：

转录是基因表达的第一步，因此非常重要。本课题组致力于构建独特的纳米芯片，阐明转录机制和细胞命运调控模式，开发新的精确控制细胞命运的手段。

课题组网站：

研究成果展示

In synthetic biology, the control of gene expression requires a multistep processing of biological signals. The key steps are sensing the environment, computing information and outputting products. To achieve such functions, the laborious, combinational networking of enzymes and substrate-genes is required, and to resolve problems, sophisticated design automation tools have been introduced. However, the complexity of genetic circuits remains low because it is difficult to completely avoid crosstalk between the circuits.

To overcome these problems, we have combined a synthetic biology approach (EMBO J. 2010) and a biophysical approach (Nature 2015, Mol Cell 2015, 2018, 2019), making a unique nano-chip by integrating an actuator (enzyme) and sensors onto a DNA origami-based nanochip (Nature Nanotechnology 2018). This gene nanochip allows us to rationally design the chemical reaction and precise gene expression control in vitro and in vivo. Our approach to component integration on a nanochip may provide a basis for large-scale, integrated genetic circuits.

代表性论文（*第一作者，#通讯作者）

1. Liu YX(刘宇翔)*; Wang RX(王瑞璇)*; 陈启明星; Chang Y(常艳); Chen Q(陈琦); Fukumoto Kodai; Wang BX(王柄巽); Yu JC(俞健晨); Luo ZF(罗长峰); Ma JY(马家媛); Chen XX(陈晓霞); Murayama Yuko; Umeda Kenichi; Kodera Noriyuki; Harada Yoshie; Sekine Shun-ichi; Li JF(李剑峰)^#; Tadakuma Hisashi^#; .Organ-specific gene expression control using DNA origami-based nanodevices.NANO LETTERS. 2024.
2. Iinuma, Ryosuke*; Chen, Xiaoxia; Masubuchi, Takeya; Ueda, Takuya; Tadakuma, Hisashi^#; .Size-Selective Capturing of Exosomes Using DNA Tripods.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY. 03 Apr 2024. 146(15):10293-10298.
3. Fukumoto, Kodai*; Miyazono, Yuya; Ueda, Takuya; Harada, Yoshie^#; Tadakuma, Hisashi^#; .Evaluating the effect of two-dimensional molecular layout on DNA origami-based transporters.NANOSCALE ADVANCES. 19 Apr 2023. 5(9):2590-2601.
4. Shuting Shen*; Masahiro Naganuma*; Yukihide Tomari; Hisashi Tadakuma^#; .Revisiting the Glass Treatment for Single-Molecule Analysis of ncRNA Function.METHODS IN MOLECULAR BIOLOGY. 08 Jul 2022. 2509:209-231.
5. Abdellatef, Shimaa A.*; Tadakuma, Hisashi; Yan, Kangmin; Fujiwara, Takashi; Fukumoto, Kodai; Kondo, Yuichi; Takazaki, Hiroko; Boudria, Rofia; Yasunaga, Takuo; Higuchi, Hideo; Hirose, Keiko^#; .Oscillatory movement of a dynein-microtubule complex crosslinked with DNA origami.ELIFE. 24 Jun 2022. 11:e76357.
6. Masahiro Naganuma*; Hisashi Tadakuma^#; Yukihide Tomari^#; .Single-molecule analysis of processive double-stranded RNA cleavage by Drosophila Dicer-2.NATURE COMMUNICATIONS. 13 Jul 2021. 12:4268.
7. Ivana Duic*; Hisashi Tadakuma; Yoshie Harada; Ryo Yamaue; Katashi Deguchi; Yuki Suzuki; Shige H. Yoshimura; Hiroki Kato; Kunio Takeyasu; Takashi Fujita^#; .Viral RNA recognition by LGP2 and MDA5, and activation of signaling through step-by-step conformational changes.NUCLEIC ACIDS RESEARCH. 18 Nov 2020. 48(20):11664-11674.
8. Yokoyama, Takeshi*; Machida, Kodai*; Iwasaki, Wakana*; Shigeta, Tomoaki; Nishimoto, Madoka; Takahashi, Mari; Sakamoto, Ayako; Yonemochi, Mayumi; Harada, Yoshie; Shigematsu, Hideki; Shirouzu, Mikako; Tadakuma, Hisashi^#; Imataka, Hiroaki^#; Ito, Takuhiro^#; .HCV IRES Captures an Actively Translating 80S Ribosome.MOLECULAR CELL. 2019. 74(6):1205+.
9. Masubuchi, Takeya*; Endo, Masayuki^#; Iizuka, Ryo; Iguchi, Ayaka; Yoon, Dong Hyun; Sekiguchi, Tetsushi; Qi, Hao; Iinuma, Ryosuke; Miyazono, Yuya; Shoji, Shuichi; Funatsu, Takashi; Sugiyama, Hiroshi^#; Harada, Yoshie; Ueda, Takuya^#; Tadakuma, Hisashi^#; .Construction of integrated gene logic-chip.NATURE NANOTECHNOLOGY. 2018. 13(10):933+.
10. Tsuboyama, Kotaro*; Tadakuma, Hisashi^#; Tomari, Yukihide^#; .Conformational Activation of Argonaute by Distinct yet Coordinated Actions of the Hsp70 and Hsp90 Chaperone Systems.MOLECULAR CELL. 2018. 70(4):722+.
11. Tadakuma, Hisashi^#*; Masubuchi, Takeya; Ueda, Takuya; .RNA Study Using DNA Nanotechnology.PROGRESS IN MOLECULAR BIOLOGY AND TRANSLATIONAL SCIENCE. 2016. 139:121-163.
12. Yao, Chunyan*; Sasaki, Hiroshi M.; Ueda, Takuya; Tomari, Yukihide^#; Tadakuma, Hisashi^#; .Single-Molecule Analysis of the Target Cleavage Reaction by the Drosophila RNAi Enzyme Complex.MOLECULAR CELL. 2015. 59(1):125-132.
13. Iwasaki, Shintaro*; Sasaki, Hiroshi M.*; Sakaguchi, Yuriko; Suzuki, Tsutomu; Tadakuma, Hisashi^#; Tomari, Yukihide^#; .Defining fundamental steps in the assembly of the Drosophila RNAi enzyme complex.NATURE. 2015. 521(7553):533-U274.
14. Miyazono, Yuya*; Hayashi, Masahito; Karagiannis, Peter; Harada, Yoshie; Tadakuma, Hisashi^#; .Strain through the neck linker ensures processive runs: a DNA-kinesin hybrid nanomachine study.EMBO JOURNAL. 2010. 29(1):93-106.