Principal investigator
Name:
Tomoyasu SugiyamaAssistant Professor , PhD, Assistant Professor
Position:
Affiliation:
School of Life Science and Technology
Honor:
Group Introduction
Research Area:
Chromosome and RNA Biology
Research Interests:
Our laboratory has been interested in biomolecular condensates, assembled by liquid-liquid phase separation. We have been studying (1) how the Cajal body, a nuclear condensate, positively regulates cellular quiescence, (2) how a nuclear RNA-binding protein that forms nuclear bodies facilitates the formation of constitutive heterochromatin, and (3) selective RNA degradation by the factors enriched in another nuclear condensate cleavage bodies. In addition, we recently started to work on the underlying mechanism of fungal pathogenicity.
Research Achievement
We previously studied how RNA interference (RNAi) directs heterochromatin assembly and a histone deacetylase complex imposes transcriptional silencing at heterochromatin. Then, we shifted our interest to biomolecular condensates in the nucleus. We have identified and characterized a critical RNA elimination complex called MTREC and EMC. Surprisingly, the RNA elimination complex turned out to prevent uniparental disomy (a condition where both copies of a chromosome come from the same parent), which could cause human diseases. Moreover, we recently demonstrated that the fission yeast Schizosaccharomyces pombe has Coilin, an integral component of the Cajal body, and revealed that Coilin is essential for cellular quiescence, a resting state in which cells do not divide but retain the capacity to revert to the cell cycle in response to appropriate stimuli. In sum, our research has contributed substantially to understanding the roles of nuclear condensates in eukaryotes.
Representative Publications (*First Author, # Corresponding Author)
- 1. Deng, Xiaoling*; Yao, Qinglian; Horvath, Attila; Jiang, Ziling; Zhao, Junjie; Fischer, Tamas; Sugiyama, Tomoyasu#; .The fission yeast ortholog of Coilin, Mug174, forms Cajal body-like nuclear condensates and is essential for cellular quiescence.NUCLEIC ACIDS RESEARCH. 01 Jun 2024.
- 2. Wang, Xindi*; Xu, Ruoming; Wang, Yichen; Liu, Ziyue; Lou, Ronghui; Sugiyama, Tomoyasu#; .Yesprit and Yeaseq: Applications for designing primers and browsing sequences for research using the four Schizosaccharomyces species.YEAST. Nov 2021. 38(11):583-591.
- 3. Marayati, Bahjat F.*; Tucker, James F.; De La Cerda, David A.; Hou, Tien-Chi; Chen, Rong; Sugiyama, Tomoyasu; Pease, James B.; Zhang, Ke#; .The Catalytic-Dependent and -Independent Roles of Lsd1 and Lsd2 Lysine Demethylases in Heterochromatin Formation in Schizosaccharomyces pombe.CELLS. Apr 2020. 9(4).
- 4. Taneja, Nitika*; Zofall, Martin; Balachandran, Vanivilasini; Thillainadesan, Gobi; Sugiyama, Tomoyasu; Wheeler, David; Zhou, Ming; Grewal, Shiv I. S.#.SNF2 Family Protein Fft3 Suppresses Nucleosome Turnover to Promote Epigenetic Inheritance and Proper Replication.Molecular Cell. 6 April 2017.
- 5. Folco, H. Diego*; Chalamcharla, Venkata R.; Sugiyama, Tomoyasu; Thillainadesan, Gobi; Zofall, Martin; Balachandran, Vanivilasini; Dhakshnamoorthy, Jothy; Mizuguchi, Takeshi; Grewal, Shiv I. S.#; .Untimely expression of gametogenic genes in vegetative cells causes uniparental disomy.NATURE. 2017. 543(7643):126+.
- 6. Sugiyama, Tomoyasu*; Thillainadesan, Gobi; Chalamcharla, Venkata R.; Meng, Zhaojing; Balachandran, Vanivilasini; Dhakshnamoorthy, Jothy; Zhou, Ming; Grewal, Shiv I. S.#; .Enhancer of Rudimentary Cooperates with Conserved RNA-Processing Factors to Promote Meiotic mRNA Decay and Facultative Heterochromatin Assembly.MOLECULAR CELL. 2016. 61(5):747-759.
- 7. Zhou, Yang*; Zhu, Jianguo; Schermann, Geza; Ohle, Corina; Bendrin, Katja; Sugioka-Sugiyama, Rie; Sugiyama, Tomoyasu#; Fischer, Tamas#; .The fission yeast MTREC complex targets CUTs and unspliced pre-mRNAs to the nuclear exosome.NATURE COMMUNICATIONS. 2015. 6.
- 8. Sugiyama, Tomoyasu#*; Wanatabe, Nobuyoshi; Kitahata, Eri; Tani, Tokio; Sugioka-Sugiyama, Rie; .Red5 and three nuclear pore components are essential for efficient suppression of specific mRNAs during vegetative growth of fission yeast.NUCLEIC ACIDS RESEARCH. 2013. 41(13):6674-6686.
- 9. Sugiyama, Tomoyasu#*; Sugioka-Sugiyama, Rie; .Red1 promotes the elimination of meiosis-specific mRNAs in vegetatively growing fission yeast.EMBO JOURNAL. 2011. 30(6):1027-1039.
- 10. Sugiyama, Tomoyasu*; Cam, Hugh P.; Sugiyama, Rie; Noma, Ken-ichi; Zofall, Martin; Kobayashi, Ryuji; Grewal, Shiv I. S.#; .SHREC, an effector complex for heterochromatic transcriptional silencing.CELL. 2007. 128(3):491-504.
- 11. Sugiyama, T*; Cam, H; Verdel, A; Moazed, D; Grewal, SIS#; .RNA-dependent RNA polymerase is an essential component of a self-enforcing loop coupling heterochromatin assembly to siRNA production.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 2005. 102(1):152-157.
- 12. Cam, HP*; Sugiyama, T; Chen, ES; Chen, X; FitzGerald, PC; Grewal, SIS#; .Comprehensive analysis of heterochromatin- and RNAi-mediated epigenetic control of the fission yeast genome.NATURE GENETICS. 2005. 37(8):809-819.
- 13. Verdel, A*; Jia, ST; Gerber, S; Sugiyama, T; Gygi, S; Grewal, SIS#; Moazed, D#; .RNAi-mediated targeting of heterochromatin by the RITS complex.SCIENCE. 2004. 303(5658):672-676.
Funding
- 1. General Program, NSFC, Leader
Group Member and Photo
-
Name：Song Xiuyi
Position：Engineer
Duration：
Email：
-
Name：Liu Ziyue
Position：Postdoct
Duration：
Email：liuzy8@shanghaitech.edu.cn
-
Name：Deng Xiaoling
Position：Doctoral Student
Duration：
Email：
-
Name：Zhao Junjie
Position：Doctoral Student
Duration：202103-Now
Email：zhaojj1@@shanghaitech.edu.cn
-
Name：Yang Rongtian
Position：Postgraduate Student
Duration：202302-Now
Email：yangrt2022@@shanghaitech.edu.cn
-
Name：Guo XinYu
Position：Research Professor
Duration：202302-Now
Email：202302-至今
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