Principal investigator
Name:
Ji-Long LiuProfessor , PhD, Professor
Position:
Vice Dean of School of Life Science and Technology
Affiliation:
School of Life Science and Technology
Honor:
Group Introduction
Research Area:
Metabolic Cell Biology, Cytoophidia
Research Interests:
The cytoophidium (plural “cytoophidia”), meaning the cellular snake in Greek, represents a novel type of organelles. In 2010, three groups including us discovered that CTP synthase is compartmentalised into the cytoophidium. Cytoophidia have existed on our planet for at least 3 billion years and are evolutionarily conserved from bacteria to human. Using the fruitfly Drosophila melanogaster, the fission yeast Schizosaccharomyces pombe and human cells as prime model systems, we will study the mechanisms by which metabolic enzymes such as CTP synthase and IMP dehydrogenase are compartmentalised within a cell. CTP synthase and IMP dehydrogenase have been attractive targets for developing agents against cancer, virus and parasites. We will investigate how various metabolic enzymes are assembled into cytoophidia and how the cytoophidium and its kind are linked to metabolic regulation and cancer biology.
Representative Publications (*First Author, # Corresponding Author)
- 1. Liu, JL*; Murphy, C; Buszczak, M; Clatterbuck, S; Goodman, R; Gall, JG#.The Drosophila melanogaster Cajal body.JOURNAL OF CELL BIOLOGY. 2006. 172(6):875-884.
- 2. Liu, Ji-Long#*.Intracellular compartmentation of CTP synthase in Drosophila.JOURNAL OF GENETICS AND GENOMICS. 2010. 37(5):281-296.
- 3. Bassett, Andrew R.#*; Tibbit, Charlotte; Ponting, Chris P.; Liu, Ji-Long#.Highly Efficient Targeted Mutagenesis of Drosophila with the CRISPR/Cas9 System.CELL REPORTS. 2013. 4(1):220-228.
- 4. Ghosh, Sanjay#*; Tibbit, Charlotte; Liu, Ji-Long#.Effective knockdown of Drosophila long non-coding RNAs by CRISPR interference.NUCLEIC ACIDS RESEARCH. 2016. 44(9).
- 5. Aughey, Gabriel N.*; Grice, Stuart J.; Liu, Ji-Long#.The Interplay between Myc and CTP Synthase in Drosophila.PLOS GENETICS. 2016. 12(2).
- 6. Liu, Ji-Long#*.The Cytoophidium and Its Kind: Filamentation and Compartmentation of Metabolic Enzymes.ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY. 2016. 32:349-372.
- 7. Huang, Yong*; Wang, Jin-Jun; Ghosh, Sanjay#; Liu, Ji-Long#.Critical roles of CTP synthase N-terminal in cytoophidium assembly.EXPERIMENTAL CELL RESEARCH. 15 May 2017. 354(2):122-133.
- 8. Chang, Chia-Chun*; Jeng, Yung-Ming; Peng, Min; Keppeke, Gerson Dierley; Sung, Li-Ying#; Liu, Ji-Long#.CTP synthase forms the cytoophidium in human hepatocellular carcinoma.EXPERIMENTAL CELL RESEARCH. 15 Dec 2017. 361(2):292-299.
- 9. Keppeke, Gerson Dierley*; Chang, Chia Chun*; Peng, Min; Chen, Li-Yu; Lin, Wei-Cheng; Pai, Li-Mei; Andrade, Luis Eduardo Coelho; Sung, Li-Ying#; Liu, Ji-Long#.IMP/GTP balance modulates cytoophidium assembly and IMPDH activity.CELL DIVISION. 15 Jun 2018. 13.
- 10. Chang, Chia-Chun*; Keppeke, Gerson D.*; Sung, Li-Ying#; Liu, Ji-Long#.Interfilament interaction between IMPDH and CTPS cytoophidia.FEBS JOURNAL. Oct 2018. 285(20):3753-3768.
- 11. Zhang, Shanshan*; Ding, Kang; Shen, Qing-Ji; Zhao, Suwen; Liu, Ji-Long#.Filamentation of asparagine synthetase in Saccharomyces cerevisiae.PLOS GENETICS. Oct 2018. 14(10).
- 12. Li, Hui*; Ye, Fangfu; Ren, Jing-Yi; Wang, Peng-Ye; Du, Li-Lin; Liu, Ji-Long#.Active transport of cytoophidia in Schizosaccharomyces pombe.FASEB JOURNAL. Nov 2018. 32(11):5891-5898.
- 13. Sun, Zhe*; Liu, Ji-Long#.mTOR-S6K1 pathway mediates cytoophidium assembly.JOURNAL OF GENETICS AND GENOMICS. Feb 2019. 46(2):65-74.
- 14. Wu, Zheng*; Liu, Ji-Long#.Cytoophidia respond to nutrient stress in Drosophila.EXPERIMENTAL CELL RESEARCH. 15 Mar 2019. 376(2):159-167.
- 15. Sun, Zhe*; Liu, Ji-Long#.Forming cytoophidia prolongs the half-life of CTP synthase.CELL DISCOVERY. 18 Jun 2019. 5.
- 16. Andreadis, Christos*; Hulme, Lydia; Wensley, Katherine; Liu, Ji-Long#.The TOR pathway modulates cytoophidium formation in Schizosaccharomyces pombe.JOURNAL OF BIOLOGICAL CHEMISTRY. 2019. 294(40):14686-14703.
- 17. Zhang, Jing*; Liu, Ji-Long#.Temperature-sensitive cytoophidium assembly in Schizosaccharomyces pombe.JOURNAL OF GENETICS AND GENOMICS. 20 Sep 2019. 46(9):423-432.
- 18. Zhou, Xian*; Guo, Chen-Jun*; Hu, Huan-Huan; Zhong, Jiale; Sun, Qianqian; Liu, Dandan; Zhou, Shuang; Chang, Chia Chun; Liu, Ji-Long#.Drosophila CTP synthase can form distinct substrate- and product-bound filaments.JOURNAL OF GENETICS AND GENOMICS. 20 Nov 2019. 46(11):537-545.
- 19. Zhang Ziheng*; Sun Weiping*; Shi Tiezhu*; Lu Pengfei#; Zhuang Min#; Liu Ji-Long#.Capturing RNA-protein Interaction via CRUIS.NUCLEIC ACIDS RESEARCH. 06 Mar 2020. 48(9):e52.
- 20. Zhang, Bo*; Tastan, Omur Y.*; Zhou, Xian*; Guo, Chen-Jun; Liu, Xuyang; Thind, Aaron; Hu, Huan-Huan; Zhao, Suwen; Liu, Ji-Long#.The proline synthesis enzyme P5CS forms cytoophidia in Drosophila.JOURNAL OF GENETICS AND GENOMICS. 20 Mar 2020. 47(3):131-143.
- 21. Zhou, Shuang*; Xiang, Hua; Liu, Ji-Long#.CTP synthase forms cytoophidia in archaea.JOURNAL OF GENETICS AND GENOMICS. 20 Apr 2020. 47(4):213-223.
- 22. Liu, Ji-Long#*; Gall, Joseph G.#.Cold shock induces novel nuclear bodies in Xenopus oocytes.EXPERIMENTAL CELL RESEARCH. Jan 2021. 398(1).
- 23. Li, Yi-Lan*; Liu, Ji-Long#.Hypoosmolality impedes cytoophidium integrity during nitrogen starvation.YEAST. Jan 2021. 38(4):276-289.
- 24. Wang, Qiao-Qi*; Zhao, Peiyao A.; Tastan, Omur Y.; Liu, Ji-Long.Polarised maintenance of cytoophidia in Drosophila follicle epithelia.EXPERIMENTAL CELL RESEARCH. 2021. 402(2).
- 25. Zhou, Xian*; Guo, Chen-Jun*; Chang, Chia-Chun*; Zhong, Jiale; Hu, Huan-Huan; Lu, Guang-Ming; Liu, Ji-Long#.Structural basis for ligand binding modes of CTP synthase.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 2021. 118(30).
- 26. Zhou, Youfang*; Liu, Jingnan; Zhang, Yuanbing; Liu, Ji-Long.Drosophila intestinal homeostasis requires CTP synthase.EXPERIMENTAL CELL RESEARCH. 2021. 408(1).
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