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Jia Chen, PhDProfessor

Tel:

Email: chenjia@@shanghaitech.edu.cn

Fax: 86-21-20685430

Add: 393 Middle Huaxia Road, Pudong, Shanghai

Faculty KMS Profile

中文信息English
Group of DNA Repair, Gene Editing & Gene Therapy

Principal investigator

Name:

Jia ChenProfessor , PhD, Professor

Position:

Affiliation:

School of Life Science and Technology

Honor:

Education Background:
  • 1998/09-2002/06, College of Life Science, Nankai University, BS
  • 2002/09-2009/04, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, PhD
Working Experience:
  • 2009/07-2014/06, National Institutes of Health, Postdoc Fellow
  • 2014/11-2019/09, School of Life Science and Technology, ShanghaiTech University, Assistant Professor (Tenure-Track)
  • 2019/10-2024/02, School of Life Science and Technology, ShanghaiTech University, Associate Professor (Tenured)
  • 2024/02-Now, School of Life Science and Technology, ShanghaiTech University, Professor (Tenured)

Group Introduction

Research Area:
DNA Repair, Gene Editing and Gene Therapy
Research Interests:

Species survival is closely related to genome stability, which is monitored and maintained by DNA repair systems. However, cell can initiate error-prone DNA repair in various physiological and pathological conditions. Thus, the fidelity of DNA repair affects disease, aging and evolution.

Genome editing is a type of genetic engineering, by which DNA is inserted, deleted or replaced in the genome of a living organism. Genome editing tools can be broadly applied in life science research and biomedicine. Though CRISPR/Cas gene editing system is efficient in gene knockout, the efficiency of CRISPR/Cas-mediated precise editing is usually low. Recently, novel gene editing systems, including base editor and prime editor, which combine nucleotide deaminase and reverse transcriptase respectively with the CRISPR/Cas system, have been developed with high editing efficiency and precision.

Our lab focuses on DNA repair-induced mutagenesis, the development and application of new gene editing systems and gene editing therapy. We are particularly interested in: 1) DNA repair-induced mutagenesis in gene editing, carcinogenesis and aging; 2) Development of new gene editing tools; 3) Gene editing therapy for human disease.


Group Website:
http://jchenlab.org/

Research Achievement

Our lab revealed the mechanism of APOBEC cytidine deaminase-mediated mutagenesis during the repair of CRISPR/Cas9-generated DNA breaks. We developed multiple series of base editors, including enhanced base editors (eBEs) with improved product purity and efficiency, dCpf1 base editors (dCpf1-BEs) for editing in A/T-rich regions, human APOBEC3A base editors (hA3A-BEs) for efficient editing in G/C-rich and/or highly methylated regions, dCas12a-derived base editors (BEACON) for efficient editing with only background levels of DNA damage response and transformer base editors (tBEs) for eliminating gRNA-dependent and gRNA-independent off-target mutations. We also determined the genome-wide and transcriptome-wide off-target effects by prime editor 3 (PE3) and developed sPE and aPE systems with improved editing efficiency.


Representative Publications (*First Author, # Corresponding Author)

Monograph

Patent

Funding

Awards

Research Achievement

Group Member and Photo




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