课题组长
姓名：
豆佳宜助理教授 研究员 博士生导师, PhD, 助理教授
职务：
所在院所：
生命科学与技术学院
荣誉称号：
教育经历：
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2006/09—2010/07，中国科学技术大学，学士
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2010/09—2017/06，华盛顿大学，博士
博士后及工作经历：
- 2017/06—2018/06，华盛顿大学，David Baker实验室，博士后
- 2018/06—2023/06，斯坦福大学，Karl Deisseroth实验室，博士后
- 2023/09—至今，上海科技大学，助理教授
课题组简介
研究内容：
我们实验室的主要目标是设计用于神经科学技术发展的工具性蛋白质。这个目标的检测性成果将是在动物大脑中实现从头设计的蛋白质功能。不同于自然界天然存在的蛋白质结构与功能的复杂性，从头设计的全新蛋白质符合结构简单功能单一的模块化要求，具有发展成为用于研究复杂生物体系的分子工具的有利条件。我们通过计算机设计自然界从未出现过的全新蛋白质并测试它们在细胞中的活性，来推进计算机设计蛋白质技术的进一步发展。我们聚焦于设计具有神经生理学功能的蛋白质，从而加速神经生物学家对大脑的解密。具体来说，我们实验室目前开展的课题有：1. 计算机设计和实验检测用于光遗传的全新光敏性蛋白质；2. 设计和优化全新门控离子通道在神经元中的表达和运输；3. 优化光控细胞膜融合工具及探索膜融合在神经细胞中的应用。
代表性论文（*第一作者，#通讯作者）
- 1. Jiayi Dou*; Anastassia A.Vorobieva*; William Sheffler; Lindsey A.Doyle; Hahnbeom Park; Matt hew J.Bick; Binchen Mao; Glenna W.Foight; Min Yen Lee; Lauren A.Gagnon; Lauren Carter; Banumathi Sankaran; Sergey Ovchinnikov; Enrique Marcos; Po-Ssu Huang; Joshua C.Vaughan; Barry L.Stoddard; David Baker#.De novo design of a fluorescence-activating β-barrel.Nature. 12 Sep, 2018.
- 2. Jiayi Dou*; Lindsey Doyle; Per Jr.Greisen; Alberto Schena; Hahnbeom Park; Kai Johnsson; Ba rry L.Stoddard; David Baker#.Sampling and energy evaluation challenges in ligand binding protein design.Protein Science. 04 Oct 2017.
- 3. Jiayi Dou*; Inna Goreshnik; Cassie Bryan; David Baker; Eva-Maria Strauch#.Parallelized Id entification of On- and Off-Target Protein Interactions.Molecular Systems Design & Engineering. 26 Nov 2019.
- 4. Xu, Chunfu*; Lu, Peilong#*; Gamal El-Din, Tamer M; Pei, Xue Y; Johnson, Matthew C; Uyeda, At suko; Bick, Matthew J; Xu, Qi; Jiang, Daohua; Bai, Hua; Reggiano, Gabriella; Hsia, Yang; Brunette , T J; Dou, Jiayi; Ma, Dan; Lynch, Eric M; Boyken, Scott E; Huang, Po-Ssu; Stewart, Lance; DiMaio , Frank; Kollman, Justin M; Luisi, Ben F; Matsuura, Tomoaki; Catterall, William A#; Baker, David#.Computational design of transmembrane pores.Nature. 26 Aug 2020.
- 5. Klima, Jason C*.; Doyle, Lindsey A.; Lee, Justin Daho; Rappleye, Michael; Gagnon, Lauren A .; Lee, Min Yen; Barros, Emilia P.; Vorobieva, Anastassia A.; Dou, Jiayi; Bremner, Samantha; Quon , Jacob S.; Chow, Cameron M.; Carter, Lauren; Mack, David L.; Amaro, Rommie E.; Vaughan, Joshua C .; Berndt, Andre; Stoddard, Barry L.; Baker, David#.Incorporation of sensing modalities into de novo designed fluorescence-activating proteins.Nature Communications. 08 Feb 2021.
- 6. Christine E.Tinberg*; Sagar D.Khare*; Jiayi Dou; Lindsey Doyle; Jorgen W.Nelson; Alberto Sc hena; Wojciech Jankowski; Charalampos G.Kalodimos; Kai Johnsson; Barry L.Stoddard; David Baker#.Computational design of ligand-binding proteins with high affinity and selectivity.Nature. 04 Sep 2013.
- 7. Enrique Marcos*; Benjamin Basanta*; Tamuka M.Chidyausiku; Yuefeng Tang; Gustav Oberdorfer; Gaohua Liu; G.V.T.Swapna; Rongjin Guan; Daniel-Adriano Silva; Jiayi Dou; Jose Henrique Pereira; R ong Xiao; Banumathi Sankaran; Peter H.Zwart; Gaetano T.Montelione; David Baker#.Principles for d esigning proteins with cavities formed by curved β sheets.Science. 13 Jan 2017.
- 8. Emilia P.Barros*; Jamie M.Schiffer; Anastassia Vorobieva; Jiayi Dou; David Baker; Rommie E .Amaro#.Improving the Efficiency of Ligand-Binding Protein Design with Molecular Dynamics.Journal of Chemical Theory and Computation. 13 Aug 2019.
- 9. Richard D.Smith;* Kelly L.Damm-Ganamet; James B.Dunbar; Aqeel Ahmed; Krishnapriya Chinnasw amy; James E.Delproposto; Ginger M.Kubish; Christine E.Tinberg; Sagar D.Khare; Jiayi Dou; Lindsey Doyle; Jeanne A.Stuckey; David Baker; Heather A.Carlson#.CSAR Benchmark Exercise 2013: Evaluatio n of Results from a Combined Computational Protein Design, Docking, and Scoring/Ranking Challenge.Journal of Chemical Information and Modeling. 29 Sep 2015.
专利
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1. David Baker, Christine Tinberg, Sargar Khare, Jiayi Dou，High Affinity Digoxigenin Binding Proteins，国际，US 9,840,539 B2，发明专利
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2. Jiayi Dou, Anastassia Vorobieva, Jason Klima, David Baker，Beta Barrel Polypeptides and Methods for Their Use，国际，US 2021/0047373 A1，发明专利
加入我们
目前实验室正在招聘一位科研助理和一位博士后（具体要求见学校招聘主页），有意向者请发送邮件至doujy@shanghaitech.edu.cn。
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