课题组长
姓名:
孙博副教授 研究员 博士生导师, PhD, 副教授
职务:
所在院所:
生命科学与技术学院
荣誉称号:
教育经历:
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2000/09—2004/06,吉林大学,学士
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2004/09—2009/06,中国科学院物理研究所,博士
博士后及工作经历:
- 2009/07—2015/06,美国康奈尔大学/霍华德休斯医学研究所,博士后
- 2015/08—2021/07,上海科技大学,生命科学与技术学院,助理教授(TENURE-TRACK)
- 2021/07—至今,上海科技大学,生命科学与技术学院,副教授(TENURED)
课题组简介
研究内容:
以应用及发展光镊、磁镊以及荧光共振能量转移等单分子技术为牵引,围绕DNA复制、修复、重组、编辑及分离等核酸代谢过程进行重要分子机器(例如:解旋酶、聚合酶、核酸酶、重组酶等)的动力学解析,理解它们的分子机制、功能及协作模式,为治疗由该类蛋白异常引起的相应疾病提供分子生物学基础。
代表性论文(*第一作者,#通讯作者)
- 1. Song, Xiaoxuan*; Chen, Ziting*; Sun, Wenjun; Yang, Hao; Guo, Lijuan; Li, Yanan; Zhao, Yilin; Ren, Zhiyun; Shi, Jin; Liu, Cong; Ma, Peixiang; Huang, Xingxu; Ji, Quanjiang; Sun, Bo#; .CRISPR-AsCas12f1 couples out-of-protospacer DNA unwinding with exonuclease activity in the sequential target cleavage.NUCLEIC ACIDS RESEARCH. 12 Nov 2024.
- 2. Li, Yanan*; Liu, Chao*; Jia, Xinshuo; Bi, Lulu; Ren, Zhiyun; Zhao, Yilin; Zhang, Xia; Guo, Lijuan; Bao, Yanling; Liu, Cong; Li, Wei#; Sun, Bo#; .RPA transforms RNase H1 to a bidirectional exonuclease for efficient RNA–DNA hybrid cleavage.NATURE COMMUNICATIONS. 29 Aug 2024. 15(1):7464.
- 3. Zhao, Yilin*; Guo, Lijuan*; Hu, Jiaojiao*; Ren, Zhiyun; Li, Yanan; Hu, Meng; Zhang, Xia; Bi, Lulu; Li, Dan; Ma, Hanhui; Liu, Cong#; Sun, Bo#; .Phase-separated ParB enforces diverse DNA compaction modes and stabilizes the parS-centered partition complex.NUCLEIC ACIDS RESEARCH. 22 Jun 2024. 52:8385-8398.
- 4. Guo, Lijuan*; Bao, Yanling*; Zhao, Yilin; Ren, Zhiyun; Bi, Lulu; Zhang, Xia; Liu, Cong; Hou, Xi-Miao; Wang, Michelle D; Sun, Bo#; .Joint efforts of replicative helicase and SSB ensure inherent replicative tolerance of G-quadruplex.ADVANCED SCIENCE. 21 Dec 2023. 11(9):2307696.
- 5. Chen, Ziting*; Li, Xiangyang*; Zhang, Qian; Sun, Wenjun; Song, Xiaoxuan; Zhang, Xia; Huang, Xingxu#; Sun, Bo#; .Enlarged DNA unwinding by Nme2Cas9 permits a broadened base editing window beyond the protospacer.SCIENCE CHINA LIFE SCIENCES. 18 Aug 2023. 67:424-427.
- 6. Liu, Chao*; Wang, Liying*; Li, Yanan*; Guo, Mengmeng; Hu, Jun; Wang, Teng; Li, Mengjing; Yang, Zhuo; Lin, Ruoyao; Xu, Wei; Chen, Yinghong; Luo, Mengcheng; Gao, Fei; Chen, Jia-Yu; Sun, Qianwen; Liu, Hongbin; Sun, Bo#; Li, Wei#; .RNase H1 facilitates recombinase recruitment by degrading DNA–RNA hybrids during meiosis.NUCLEIC ACIDS RESEARCH. 28 Jun 2023. 51(14):7357-7375.
- 7. Jia, Xinshuo*; Li, Yanan*; Wang, Teng; Bi, Lulu; Guo, Lijuan; Chen, Ziting; Zhang, Xia; Ye, Shasha; Chen, Jia; Yang, Bei; Sun, Bo#; .Discrete RNA–DNA hybrid cleavage by the EXD2 exonuclease pinpoints two rate-limiting steps.THE EMBO JOURNAL. 04 Jan 2023. 42:e111703.
- 8. Wang, Teng*; Hu, Jiaojiao*; Li, Yanan; Bi, Lulu; Guo, Lijuan; Jia, Xinshuo; Zhang, Xia; Li, Dan; Hou, Xi-Miao; Modesti, Mauro; Xi, Xu-Guang; Liu, Cong#; Sun, Bo#; .Bloom syndrome helicase compresses single-stranded DNA into phase-separated condensates.ANGEWANDTE CHEMIE INTERNATIONAL EDITION. 26 Sep 2022. 61:e202209463.
- 9. Guo, Lijuan*; Zhao, Yilin*; Zhang, Qian; Feng, Ying; Bi, Lulu; Zhang, Xia; Wang, Teng; Liu, Cong; Ma, Hanhui; Sun, Bo#; .Stochastically multimerized ParB orchestrates DNA assembly as unveiled by single-molecule analysis.NUCLEIC ACIDS RESEARCH. 09 Sep 2022. 50(16):9294-9305.
- 10. Bi, Lulu*; Qin, Zhenheng*; Wang, Teng; Li, Yanan; Jia, Xinshuo; Zhang, Xia; Hou, Xi-Miao; Modesti, Mauro; Xi, Xu-Guang; Sun, Bo#; .The convergence of head-on DNA unwinding forks induces helicase oligomerization and activity transition.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 03 Jun 2022. 119(23):e2116462119.
- 11. Zhang, Qian*; Chen, Ziting*; Wang, Fangzhu; Zhang, Siqi; Chen, Hongyu; Gu, Xueying; Wen, Fengcai; Jin, Jiachuan; Zhang, Xia; Huang, Xingxu; Shen, Bin#; Sun, Bo#; .Efficient DNA interrogation of SpCas9 governed by its electrostatic interaction with DNA beyond the PAM and protospacer.NUCLEIC ACIDS RESEARCH. 02 Dec 2021. 49(21):12433-12444.
- 12. Ye, Shasha*; Chen, Ziting*; Zhang, Xia; Li, Fangfang; Guo, Lijuan; Hou, Xi-Miao; Wu, Wen-Qiang; Wang, Jian; Liu, Cong; Zheng, Ke; Sun, Bo#; .Proximal Single-Stranded RNA Destabilizes Human Telomerase RNA G-Quadruplex and Induces Its Distinct Conformers.THE JOURNAL OF PHYSICAL CHEMISTRY LETTERS. 08 Apr 2021. 12(13):3361-3366.
- 13. Zhang, Siqi*; Zhang, Qian*; Hou, Xi-Miao; Guo, Lijuan; Wang, Fangzhu; Bi, Lulu; Zhang, Xia; Li, Hai-Hong; Wen, Fengcai; Xi, Xu-Guang; Huang, Xingxu; Shen, Bin; Sun, Bo#; .Dynamics of Staphylococcus aureus Cas9 in DNA target association and dissociation.EMBO REPORTS. 05 Oct 2020. 21(10):e50184.
- 14. Qin, Zhenheng*; Bi, Lulu*; Hou, Xi-Miao; Zhang, Siqi; Zhang, Xia; Lu, Ying; Li, Ming; Modesti, Mauro; Xi, Xu-Guang#; Sun, Bo#; .Human RPA activates BLM's bidirectional DNA unwinding from a nick.ELIFE. 26 Feb 2020. 9:e54098.
- 15. Zhang, Qian*; Wen, Fengcai*; Zhang, Siqi; Jin, Jiachuan; Bi, Lulu; Lu, Ying; Li, Ming; Xi, Xu-Guang; Huang, Xingxu; Shen, Bin#; Sun, Bo#; .The post-PAM interaction of RNA-guided spCas9 with DNA dictates its target binding and dissociation.SCIENCE ADVANCES. 13 Nov 2019. 5(11):eaaw9807.
- 16. Zhang, Xia*; Yu, Lina*; Ye, Shasha*; Xie, Jie; Huang, Xingxu; Zheng, Ke#; Sun, Bo#; .MOV10L1 binds RNA G-Quadruplex in a structure-specific manner and resolves it more efficiently than MOV10.ISCIENCE. 26 Jul 2019. 17:36-48.
- 17. Sun, Bo#*; Singh, Anupam; Sultana, Shemaila; Inman, James T.; Patel, Smita S.; Wang, Michelle D.#; .Helicase promotes replication re-initiation from an RNA transcript.NATURE COMMUNICATIONS. 13 Jun 2018. 9:2306.
- 18. Sun, Bo*; Pandey, Manjula; Inman, James T.; Yang, Yi; Kashlev, Mikhail; Patel, Smita S.; Wang, Michelle D.#; .T7 replisome directly overcomes DNA damage.NATURE COMMUNICATIONS. 17 Dec 2015. 6:10260.
- 19. Sun, Bo*; Johnson, Daniel S*; Patel, Gayatri; Smith, Benjamin Y; Pandey, Manjula; Patel, Smita S#; Wang, Michelle D#; .ATP-induced helicase slippage reveals highly coordinated subunits.NATURE. 18 Sep 2011. 478:132-135.
- 20. Sun, Bo*; Wei, Kong-Ji; Zhang, Bo; Zhang, Xing-Hua; Dou, Shuo-Xing; Li, Ming#; Xi, Xu Guang#; .Impediment of E. coli UvrD by DNA-destabilizing force reveals a strained-inchworm mechanism of DNA unwinding.THE EMBO JOURNAL. 13 Nov 2008. 27(24):3279-3287.
专著
Bi, Lulu; Qin, Zhenheng; Hou, Xi-Miao; Modesti, Mauro; Sun, Bo. Simultaneous Mechanical and Fluorescence Detection of Helicase-Catalyzed DNA unwinding (Book Chapter). OPTICAL TWEEZERS: METHODS AND PROTOCOLS. Springer, 2022.
项目
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1. I-motif结构调控DNA复制的单分子动力学研究,国家自然科学基金委面上项目,项目负责人
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2. 多态帕金森淀粉样纤维的单分子研究,上海科技大学生命学院发展基金,项目负责人
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3. 解析RNA–DNA杂合体调控同源重组的动力学分子机制,上海市自然科学基金面上项目,项目负责人
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4. 极小型CRISPR核酸酶Cas12f的单分子动力学解析及优化,国家自然科学基金委面上项目,项目负责人
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5. 单分子技术解析G四链体调控DNA复制分子机制,上海市自然科学基金面上项目,项目负责人
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6. 单分子技术解析BLM解旋酶在DNA同源重组修复中的分子机制及功能,国家自然科学基金委青年科学基金项目,项目负责人
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7. 核酸互作蛋白的单分子动力学解析,国家自然科学基金委优秀青年科学基金项目,项目负责人
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8. 单分子技术研究MOV10解旋酶分子机制,上海市自然科学基金面上项目,项目负责人
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9. lncRNA甲基化修饰在多能干细胞维持与分化中的作用及机制研究,科技部国家重点研发计划青年项目,课题负责人
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10. RNA结合蛋白在精子发生过程中的作用,科技部国家重点研发计划,课题参与人员(非负责人)
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11. 单分子技术研究核酸酶在DNA修复过程中的功能机制,上海市浦江人才计划,项目负责人
奖励
- 1. 2008年,中国科学院物理研究所所长奖学金
- 2. 2016年,上海市浦江人才
- 3. 2020年,国家自然科学基金优秀青年科学基金
- 4. 2020年,上海科技大学优秀教师
- 5. 2021年,中国生物物理学会单分子生物学分会委员
- 6. 2021年,上海科技大学优秀教师
- 7. 2022年,上海科技大学优秀教师
课题组成员及合影
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姓名:张霞
身份:高级工程师
在组时间:2015/12-至今
邮箱:zhangxia@@shanghaitech.edu.cn
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姓名:任知韵
身份:博士研究生
在组时间:2021/01-至今
邮箱:renzhy1@@shanghaitech.edu.cn
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姓名:胡萌
身份:博士研究生
在组时间:2022/11-至今
邮箱:humeng@@shanghaitech.edu.cn
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姓名:鲍彦伶
身份:博士研究生
在组时间:2022/01-至今
邮箱:baoyl@@shanghaitech.edu.cn
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姓名:宋晓萱
身份:博士研究生
在组时间:2022/01-至今
邮箱:songxx@@shanghaitech.edu.cn
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姓名:程昺凯
身份:博士研究生
在组时间:2023/01-至今
邮箱:chengbk2022@@shanghaitech.edu.cn
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姓名:陈丽双
身份:博士研究生
在组时间:2023/01-至今
邮箱:chenlsh2022@@shanghaitech.edu.cn
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姓名:赵毅
身份:硕士研究生
在组时间:2024/01-至今
邮箱:zhaoyi12023@@shanghaitech.edu.cn
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姓名:吴绍娟
身份:硕士研究生
在组时间:2024/01-至今
邮箱:wushj2023@@shanghaitech.edu.cn
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姓名:张雨婷
身份:硕士研究生
在组时间:2025/01-至今
邮箱:zhangyt2024@@shanghaitech.edu.cn
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