孙博, PhD副教授研究员博士生导师

电话： 86-21-20684536

邮箱： sunbo@@shanghaitech.edu.cn

传真： 86-21-20685430

地址：上海市浦东新区华夏中路393号

KMS个人画像

中文信息English

单分子酶学研究组

课题组长

姓名：

孙博副教授研究员博士生导师, PhD, 副教授

职务：

所在院所：

生命科学与技术学院

荣誉称号：

教育经历：

2000/09—2004/06，吉林大学，学士
2004/09—2009/06，中国科学院物理研究所，博士

博士后及工作经历：

2009/07—2015/06，美国康奈尔大学/霍华德休斯医学研究所，博士后
2015/08—2021/07，上海科技大学，生命科学与技术学院，助理教授（TENURE-TRACK）
2021/07—至今，上海科技大学，生命科学与技术学院，副教授（TENURED）

课题组简介

研究方向：

单分子酶学，单分子生物物理学

研究内容：

以应用及发展光镊、磁镊以及荧光共振能量转移等单分子技术为牵引，围绕DNA复制、修复、重组、编辑及分离等核酸代谢过程进行重要分子机器（例如：解旋酶、聚合酶、核酸酶、重组酶等）的动力学解析，理解它们的分子机制、功能及协作模式，为治疗由该类蛋白异常引起的相应疾病提供分子生物学基础。

课题组网站：

http://sunlab.slst.shanghaitech.edu.cn/

研究成果展示

1. 2026年1月22日，课题组在学术期刊《德国应用化学》（Angewandte Chemie International Edition）发表研究论文，证实特殊DNA二级结构G-四链体和i-Motif能够在同一条双链DNA共存，并揭示致癌剪切因子PCBP2协助复制体克服这一复合结构的新通路。

图1：iM:G4结构的动态变化及PCBP2介导的复制耐受机制。

2. 2026年2月18日，课题组与合作者在学术期刊《细胞》（Cell）发表研究论文，成功开发了一种力感应受体工程改造方法，为TCR-T细胞疗法的优化升级提供了重要支撑，也为肿瘤免疫治疗、干细胞研究以及抗体药物研发提供了新的思路。

图2：单分子光镊技术检测TCR-pMHC逆锁键强度。

3. 2026年2月27日，课题组在《美国科学院院刊》（PNAS）发表研究论文，在单分子水平揭示减数分裂重组中的特异重组酶DMC1在供体双链DNA上进行一维滑动扫描，并在辅助因子H2M1的帮助下，精准识别同源序列，完善了真核生物同源重组理论框架。

图3：重组酶DMC1在H2M1的帮助下精准识别同源序列模型。

4. 2026年4月11日，课题组与合作者在《自然-通讯》（Nature Communicaitons）发表研究论文，通过监测Hippo通路转录因子TEAD4结合DNA的动态过程，揭示其辅因子VGLL4通过调控TEAD4多聚，以剂量依赖的方式招募下游共激活因子YAP。

图4: VGLL4调控Hippo转录因子TEAD4结合DNA及招募YAP模型。

5. 2026年5月29日，课题组与合作者在《分子细胞》（Molecular Cell）发表论文，报道细菌防御系统DdmDE中的pAgo蛋白DdmE与辅助蛋白DdmD协同工作，识别、降解入侵质粒的动力学分子机制，为理解细菌免疫多样性及基因工具开发提供了理论支撑。

图5: 细菌防御系统DdmDE识别、降解入侵质粒模型。

6. 2026年6月，课题组与合作者在《核酸研究》（Nucleic Acids Research）杂志发表论文，报道在DNA双链断裂修复路径中，RPA通过与ssDNA的共相分离来富集下游重组酶RAD51，为理解同源重组依赖的DNA损伤修复提供了新见解。

图6：ssDNA的构建及RPA-ssDNA相分离富集RAD51模型。

代表性论文（*第一作者，#通讯作者）

1. Li, Yanan*; Zhao, Yi*; Chen, Yinghong*; Wang, Teng; Bi, Lulu; Bao, Yanling; Chen, Lishuang; Zhang, Xia; Cheng, Bingkai; Hu, Meng; Jing, Shengli; Liu, Chao^#; Li, Wei^#; Sun, Bo^#.RPA–ssDNA co-phase separation facilitates RAD51 enrichment during homologous recombination.NUCLEIC ACIDS RESEARCH. 20 Jun 2026. 54:gkag586.
2. Yang, Hao*; Song, Xiaoxuan*; Zhao, Jiazheng*; Zhang, Yuting; Ren, Zhiyun; Bao, Yanling; Chen, Lishuang; Hu, Meng; Cheng, Bingkai; Wu, Shaojuan; Zhao, Yi; Liu, Cong; Sun, Yadong^#; Feng, Yan^#; Sun, Bo^#.The DdmDE defense system eradicates plasmids by target-centered bidirectional ssDNA loop extrusion and site-specific cleavage.MOLECULAR CELL. 29 May 2026. 86(12):1-15.
3. Ren, Zhiyun*; Zhao, Yilin*; Yu, Wentao; Zhang, Xia; Song, Xiaoxuan; Bao, Yanling; Hu, Meng; Chen, Lishuang; Yang, Hao; Cheng, Bingkai; Liu, Cong; Jin, Yunyun^#; Zhang, Lei^#; Sun, Bo^#.VGLL4-driven TEAD4 multimerization orchestrates DNA binding and YAP recruitment.NATURE COMMUNICATIONS. 11 Apr 2026. 17(1).
4. Cheng, Bingkai*; Li, Yanan; Zhao, Yi; Zhang, Yuting; Zhang, Xia; Chen, Lishuang; Yang, Hao; Song, Xiaoxuan; Ren, Zhiyun; Liu, Cong; Xue, Jingdong; Li, Bing; Liu, Chao; Li, Wei; Sun, Bo^#.HOP2–MND1 chaperones a diffusing DMC1–ssDNA complex to survey dsDNA for homology recognition during meiotic recombination.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 27 Feb 2026. 123(9):e2529249123.
5. Wang, Yuanhao*; Wang, Yuhan*; Yuan, Wenjie*; Wang, Xiaojing*; Wang, Anhui*; Bao, Yanling*; Zhang, Yajing*; Tan, Jia Chi*; Wang, Jianglai; Liu, Junshuang; Huang, Tianqi; Han, Zixuan; Pei, Biling; Chen, Lijuan; Ren, Zhengxu; Wang, Xueqing; Hu, Lanxin; Wu, Siqi; Pang, Mengke; Wang, Sifan; Yang, Zihuan; Li, Jiaze; Huang, Delian; Shao, Shuai; Yuan, Huairui; Wu, Ling^#; Feng, Yinnian^#; Zhou, Penghui^#; Li, Guohui^#; Sun, Bo^#; Xu, Chenqi^#; Gascoigne, Nicholas^#; Zhao, Xiang^#.Tuning the sensitivity of mechanosensory receptors through histidine scanning.CELL. 18 Feb 2026. 189(6):1-21.
6. Bao, Yanling*; Wu, Shaojuan*; Guo, Lijuan; Song, Xiaoxuan; Ren, Zhiyun; Zhao, Jiazheng; Wang, Mingyao; Chen, Lishuang; Cheng, Bingkai; Hou, Xi-Miao; Liu, Cong; Sun, Yadong; Sun, Bo^#.Coexistence of G-Quadruplex and i-Motif Within a DNA Duplex is Tolerated by a PCBP2-Assisted Replisome.ANGEWANDTE CHEMIE INTERNATIONAL EDITION. 01 Dec 2025. 65(4):e202523769.
7. Bi, Lulu*; Li, Linge*; Li, Xiang*; Wu, Shaojuan; Zhang, Xia; Zhao, Yilin; Li, Dan; Liu, Cong^#; Hou, Zhonghuai^#; Sun, Bo^#.Bridging mechanical properties with atomic structures of polymorphic α-synuclein fibrils by single-molecule analysis and molecular dynamics simulations.AGGREGATE. May 2025. 6(5):e70023.
8. Li, Xiang*; Bi, Lulu*; Zhang, Shenqing; Xu, Qianhui; Xia, Wencheng; Tao, Youqi; Wu, Shaojuan; Li, Yanan; Le, Weidong; Kang, Wenyan; Li, Dan; Sun, Bo^#; Liu, Cong^#.Single-molecule insight into α-synuclein fibril structure and mechanics modulated by chemical compounds.ADVANCED SCIENCE. Apr 2025. 12(14):2416721.
9. Song, Xiaoxuan*; Chen, Ziting*; Sun, Wenjun; Yang, Hao; Guo, Lijuan; Li, Yanan; Zhao, Yilin; Ren, Zhiyun; Shi, Jin; Liu, Cong; Ma, Peixiang; Huang, Xingxu; Ji, Quanjiang; Sun, Bo^#.CRISPR-AsCas12f1 couples out-of-protospacer DNA unwinding with exonuclease activity in the sequential target cleavage.NUCLEIC ACIDS RESEARCH. 12 Nov 2024. 52(22):14030-14042.
10. Li, Yanan*; Liu, Chao*; Jia, Xinshuo; Bi, Lulu; Ren, Zhiyun; Zhao, Yilin; Zhang, Xia; Guo, Lijuan; Bao, Yanling; Liu, Cong; Li, Wei^#; Sun, Bo^#.RPA transforms RNase H1 to a bidirectional exonuclease for efficient RNA–DNA hybrid cleavage.NATURE COMMUNICATIONS. 29 Aug 2024. 15(1):7464.
11. Zhao, Yilin*; Guo, Lijuan*; Hu, Jiaojiao*; Ren, Zhiyun; Li, Yanan; Hu, Meng; Zhang, Xia; Bi, Lulu; Li, Dan; Ma, Hanhui; Liu, Cong^#; Sun, Bo^#.Phase-separated ParB enforces diverse DNA compaction modes and stabilizes the parS-centered partition complex.NUCLEIC ACIDS RESEARCH. 22 Jun 2024. 52(14):8385-8398.
12. Guo, Lijuan*; Bao, Yanling*; Zhao, Yilin; Ren, Zhiyun; Bi, Lulu; Zhang, Xia; Liu, Cong; Hou, Xi-Miao; Wang, Michelle D; Sun, Bo^#.Joint efforts of replicative helicase and SSB ensure inherent replicative tolerance of G-quadruplex.ADVANCED SCIENCE. 21 Dec 2023. 11(9):2307696.
13. Chen, Ziting*; Li, Xiangyang*; Zhang, Qian; Sun, Wenjun; Song, Xiaoxuan; Zhang, Xia; Huang, Xingxu^#; Sun, Bo^#.Enlarged DNA unwinding by Nme2Cas9 permits a broadened base editing window beyond the protospacer.SCIENCE CHINA LIFE SCIENCES. 18 Aug 2023. 67(2):424-427.
14. Liu, Chao*; Wang, Liying*; Li, Yanan*; Guo, Mengmeng; Hu, Jun; Wang, Teng; Li, Mengjing; Yang, Zhuo; Lin, Ruoyao; Xu, Wei; Chen, Yinghong; Luo, Mengcheng; Gao, Fei; Chen, Jia-Yu; Sun, Qianwen; Liu, Hongbin; Sun, Bo^#; Li, Wei^#.RNase H1 facilitates recombinase recruitment by degrading DNA–RNA hybrids during meiosis.NUCLEIC ACIDS RESEARCH. 28 Jun 2023. 51(14):7357-7375.
15. Jia, Xinshuo*; Li, Yanan*; Wang, Teng; Bi, Lulu; Guo, Lijuan; Chen, Ziting; Zhang, Xia; Ye, Shasha; Chen, Jia; Yang, Bei; Sun, Bo^#.Discrete RNA–DNA hybrid cleavage by the EXD2 exonuclease pinpoints two rate-limiting steps.THE EMBO JOURNAL. 04 Jan 2023. 42(1):e111703.
16. Wang, Teng*; Hu, Jiaojiao*; Li, Yanan; Bi, Lulu; Guo, Lijuan; Jia, Xinshuo; Zhang, Xia; Li, Dan; Hou, Xi-Miao; Modesti, Mauro; Xi, Xu-Guang; Liu, Cong^#; Sun, Bo^#.Bloom syndrome helicase compresses single-stranded DNA into phase-separated condensates.ANGEWANDTE CHEMIE INTERNATIONAL EDITION. 26 Sep 2022. 61(39):e202209463.
17. Guo, Lijuan*; Zhao, Yilin*; Zhang, Qian; Feng, Ying; Bi, Lulu; Zhang, Xia; Wang, Teng; Liu, Cong; Ma, Hanhui; Sun, Bo^#.Stochastically multimerized ParB orchestrates DNA assembly as unveiled by single-molecule analysis.NUCLEIC ACIDS RESEARCH. 09 Sep 2022. 50(16):9294-9305.
18. Bi, Lulu*; Qin, Zhenheng*; Wang, Teng; Li, Yanan; Jia, Xinshuo; Zhang, Xia; Hou, Xi-Miao; Modesti, Mauro; Xi, Xu-Guang; Sun, Bo^#.The convergence of head-on DNA unwinding forks induces helicase oligomerization and activity transition.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 03 Jun 2022. 119(23):e2116462119.
19. Zhang, Qian*; Chen, Ziting*; Wang, Fangzhu; Zhang, Siqi; Chen, Hongyu; Gu, Xueying; Wen, Fengcai; Jin, Jiachuan; Zhang, Xia; Huang, Xingxu; Shen, Bin^#; Sun, Bo^#.Efficient DNA interrogation of SpCas9 governed by its electrostatic interaction with DNA beyond the PAM and protospacer.NUCLEIC ACIDS RESEARCH. 02 Dec 2021. 49(21):12433-12444.
20. Ye, Shasha*; Chen, Ziting*; Zhang, Xia; Li, Fangfang; Guo, Lijuan; Hou, Xi-Miao; Wu, Wen-Qiang; Wang, Jian; Liu, Cong; Zheng, Ke; Sun, Bo^#.Proximal single-stranded RNA destabilizes human telomerase RNA G-quadruplex and induces its distinct conformers.THE JOURNAL OF PHYSICAL CHEMISTRY LETTERS. 08 Apr 2021. 12(13):3361-3366.
21. Zhang, Siqi*; Zhang, Qian*; Hou, Xi-Miao; Guo, Lijuan; Wang, Fangzhu; Bi, Lulu; Zhang, Xia; Li, Hai-Hong; Wen, Fengcai; Xi, Xu-Guang; Huang, Xingxu; Shen, Bin; Sun, Bo^#.Dynamics of Staphylococcus aureus Cas9 in DNA target association and dissociation.EMBO REPORTS. 05 Oct 2020. 21(10):e50184.
22. Qin, Zhenheng*; Bi, Lulu*; Hou, Xi-Miao; Zhang, Siqi; Zhang, Xia; Lu, Ying; Li, Ming; Modesti, Mauro; Xi, Xu-Guang^#; Sun, Bo^#.Human RPA activates BLM's bidirectional DNA unwinding from a nick.ELIFE. 26 Feb 2020. 9:e54098.
23. Zhang, Qian*; Wen, Fengcai*; Zhang, Siqi; Jin, Jiachuan; Bi, Lulu; Lu, Ying; Li, Ming; Xi, Xu-Guang; Huang, Xingxu; Shen, Bin^#; Sun, Bo^#.The post-PAM interaction of RNA-guided spCas9 with DNA dictates its target binding and dissociation.SCIENCE ADVANCES. 13 Nov 2019. 5(11):eaaw9807.
24. Zhang, Xia*; Yu, Lina*; Ye, Shasha*; Xie, Jie; Huang, Xingxu; Zheng, Ke^#; Sun, Bo^#.MOV10L1 binds RNA G-Quadruplex in a structure-specific manner and resolves it more efficiently than MOV10.ISCIENCE. 26 Jul 2019. 17:36-48.
25. Sun, Bo*^#; Singh, Anupam; Sultana, Shemaila; Inman, James T.; Patel, Smita S.; Wang, Michelle D.^#.Helicase promotes replication re-initiation from an RNA transcript.NATURE COMMUNICATIONS. 13 Jun 2018. 9(1):2306.
26. Sun, Bo*; Pandey, Manjula; Inman, James T.; Yang, Yi; Kashlev, Mikhail; Patel, Smita S.; Wang, Michelle D.^#.T7 replisome directly overcomes DNA damage.NATURE COMMUNICATIONS. 17 Dec 2015. 6:10260.
27. Sun, Bo*; Johnson, Daniel S*; Patel, Gayatri; Smith, Benjamin Y; Pandey, Manjula; Patel, Smita S^#; Wang, Michelle D^#.ATP-induced helicase slippage reveals highly coordinated subunits.NATURE. 18 Sep 2011. 478:132-135.
28. Sun, Bo*; Wei, Kong-Ji; Zhang, Bo; Zhang, Xing-Hua; Dou, Shuo-Xing; Li, Ming^#; Xi, Xu Guang^#.Impediment of E. coli UvrD by DNA-destabilizing force reveals a strained-inchworm mechanism of DNA unwinding.THE EMBO JOURNAL. 13 Nov 2008. 27(24):3279-3287.

专著

Bi, Lulu; Qin, Zhenheng; Hou, Xi-Miao; Modesti, Mauro; Sun, Bo. Simultaneous Mechanical and Fluorescence Detection of Helicase-Catalyzed DNA unwinding (Book Chapter). OPTICAL TWEEZERS: METHODS AND PROTOCOLS. Springer, 2022.