中文信息

干细胞与神经生物学研究组

课题组长

姓名：

向阳飞助理教授研究员博士生导师, PhD, 助理教授

职务：

所在院所：

生命科学与技术学院

荣誉称号：

教育经历：

2002/09—2006/06，怀化学院，学士
2006/09—2009/06，暨南大学，硕士
2009/09—2013/06，暨南大学，博士

博士后及工作经历：

2013/08—2019/05，耶鲁大学，博士后
2019/05—2020/02，耶鲁大学，副研究员
2020/03—至今，上海科技大学，生命科学与技术学院，助理教授 (TENURE-TRACK)

课题组简介

研究方向：

干细胞与神经生物学、类器官

研究内容：

1）神经类器官构建；2）基于类器官技术的疾病模拟与药物发现探索。

课题组网站：www.yxlab.org

欢迎生物背景、或生信背景的新成员加入我们课题组！

课题组网站：

研究成果展示

脑类器官是体外模拟大脑结构、功能、以及发育过程的三维培养物，其建立为研究人类大脑提供了简便途径，使得许多曾经难以开展的研究成为现实。利用人类多能干细胞（包括人类胚胎干细胞和诱导多能干细胞），本课题组已建立多种脑类器官模型，并以此研究不同大脑区域发育的差异调控、以及人类神经系统疾病机制与药物发现等；通过融合区域化脑类器官，我们成功在体外重构和研究大脑内区域间的连接与机制。

1）建立皮质、腹侧端脑特异类器官；构建融合培养类器官（组装体），模拟人类中间神经元迁移（Cell Stem Cell 2017）。

2）建立丘脑特异类器官；模拟丘脑与皮层间轴突发育（Cell Stem Cell 2019）。

3）脑类器官血管化构建（Nature Methods 2019）。

4）利用脑类器官模拟并研究神经发育疾病Rett Syndrome机制（Molecular Cell 2020）。

5）课题组采访介绍（Cell Stem Cell 2020）。

6）课题组采访介绍（Cell Stem Cell 2021）。

7）建立具备核团特征的腹侧丘脑类器官，模拟核团特异的发育异常（Cell Stem Cell 2023）。

课题研究相关关注报道。

开设课程：1）再生医学原理（本科生、研究生）

2）类脑器官（本科生）

参与课程：1）生物医学工程基础

2）实验生物学

代表性论文（*第一作者，#通讯作者）

1. Kiral FR*, Cakir B, Tanaka Y, Kim J, Yang WS, Wehbe F, Kang YJ, Zhong M, Sancer G, Lee SH, Xiang Y^#, Park IH^#.Generation of ventralized human thalamic organoids with thalamic reticular nucleus.Cell Stem Cell. 2023 Mar 28. 30(5):677-688.e5. DOI: 10.1016/j.stem.2023.03.007.
2. Barral, Serena^#*; Xiang, Yangfei^#; Birey, Fikri^#.Improving in vitro modeling of human brain with future brain organoids.FRONTIERS IN MOLECULAR NEUROSCIENCE. 09 Aug 2022. 15.
3. Lu, Xiaoxiang*; Yang, Jiajie; Xiang, Yangfei.Modeling human neurodevelopmental diseases with brain organoids.CELL REGENERATION. Jan 2022. 11(1).
4. Anastasia Tikhonova^#*; Yangfei Xiang^#*; Casey Gifford^#*.Early-career researchers in the time of COVID-19: Starting a new lab during a pandemic.CELL STEM CELL. 06 May 2021.
5. Xiang, Yangfei*.Human brain organoids: development and application.CANCER SCIENCE. 01 Feb 2022. 113.
6. Yoshiaki Tanaka*; Bilal Cakir; Yangfei Xiang; Gareth J. Sullivan; In-Hyun Park^#.Synthetic Analyses of Single-Cell Transcriptomes From Multiple Brain Organoids and Fetal Brain.CELL REPORTS. 2020. 30(6):1682-1689.
7. Smith, Quinton^#*; McKinley, Kara L.^#*; Chan, Chii Jou^#*; Sikandar, Shaheen^#*; Xiang, Yangfei^#*; Passaro, Diana^#*.Introductions to the Community: Early-Career Researchers in the Time of COVID-19.CELL STEM CELL. Nov 2020. 27(5):702-704.
8. Lim, Yolpanhchana*; Beane-Ebel, Jennifer E.; Tanaka, Yoshiaki; Ning, Boting; Husted, Christopher R.; Henderson, David C.; Xiang, Yangfei; Park, In-Hyun; Farrer, Lindsay A.; Zhang, Huiping^#.Exploration of alcohol use disorder-associated brain miRNA-mRNA regulatory networks.TRANSLATIONAL PSYCHIATRY. 02 Oct 2021. 11(1).
9. Xiang, Yangfei*; Tanaka, Yoshiaki*; Patterson, Benjamin; Hwang, Sung-Min; Hysolli, Eriona; Cakir, Bilal; Kim, Kun-Yong; Wang, Wanshan; Kang, Young-Jin; Clement, Ethan M.; Zhong, Mei; Lee, Sang-Hun; Cho, Yee Sook; Patra, Prabir; Sullivan, Gareth J.; Weissman, Sherman M.; Park, In-Hyun^#.Dysregulation of BRD4 Function Underlies the Functional Abnormalities of MeCP2 Mutant Neurons.MOLECULAR CELL. 2020. 79(1):84-+.
10. Yangfei Xiang^#*; Bilal Cakir; In-Hyun Park^#.Deconstructing and Reconstructing the Human Brain With Regionally Specified Brain Organoids.SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY. 2020.
11. Cakir, Bilal*; Xiang, Yangfei*; Tanaka, Yoshiaki; Kural, Mehmet H.; Parent, Maxime; Kang, Young-Jin; Chapeton, Kayley; Patterson, Benjamin; Yuan, Yifan; He, Chang-Shun; Raredon, Micha Sam B.; Dengelegi, Jake; Kim, Kun-Yong; Sun, Pingnan; Zhong, Mei; Lee, Sangho; Patra, Prabir; Hyder, Fahmeed; Niklason, Laura E.; Lee, Sang-Hun; Yoon, Young-Sup; Park, In-Hyun^#.Engineering of human brain organoids with a functional vascular-like system.NATURE METHODS. 2019. 16(11):1169.
12. Xiang, Yangfei^#*; Bilal Cakir; In-Hyun Park.Generation of regionally specified human brain organoids resembling thalamus development.CELL PRESS: STAR PROTOCOLS. 2019.
13. Xiang, Yangfei*; Tanaka, Yoshiaki*; Cakir, Bilal; Patterson, Benjamin; Kim, Kun-Yong; Sun, Pingnan; Kang, Young-Jin; Zhong, Mei; Liu, Xinran; Patra, Prabir; Lee, Sang-Hun; Weissman, Sherman M.; Park, In-Hyun^#.hESC-Derived Thalamic Organoids Form Reciprocal Projections When Fused with Cortical Organoids.CELL STEM CELL. 2019. 24(3):487.
14. Xiang, Yangfei*; Tanaka Yoshiaki; Benjamin Patterson; Bilal Cakir; Kun-Yong Kim; Yee Sook Cho; In-Hyun Park^#.Generation and fusion of human cortical and medial ganglionic eminence brain organoids.CURRENT PROTOCOLS IN STEM CELL BIOLOGY. 2018.
15. Kim, Kun-Yong*; Tanaka, Yoshiaki; Su, Juan; Cakir, Bilal; Xiang, Yangfei; Patterson, Benjamin; Ding, Junjun; Jung, Yong-Wook; Kim, Ji-Hyun; Hysolli, Eriona; Lee, Haelim; Dajani, Rana; Kim, Jonghwan; Zhong, Mei; Lee, Jeong-Heon; Skalnik, David; Lim, Jeong Mook; Sullivan, Gareth J.; Wang, Jianlong; Park, In-Hyun^#.Uhrf1 regulates active transcriptional marks at bivalent domains in pluripotent stem cells through Setd1a.NATURE COMMUNICATIONS. 2018. 9.
16. Xiang, Yangfei*; Tanaka, Yoshiaki*; Patterson, Benjamin; Kang, Young-Jin; Govindaiah, Gubbi; Roselaar, Naomi; Cakir, Bilal; Kim, Kun-Yong; Lombroso, Adam P.; Hwang, Sung-Min; Zhong, Mei; Stanley, Edouard G.; Elefanty, Andrew G.; Naegele, Janice R.; Lee, Sang-Hun; Weissman, Sherman M.; Park, In-Hyun^#.Fusion of Regionally Specified hPSC-Derived Organoids Models Human Brain Development and Interneuron Migration.CELL STEM CELL. 2017. 21(3):383.
17. Xiang, Yangfei*; Kim, Kun-Yong; Gelernter, Joel; Park, In-Hyun^#; Zhang, Huiping^#.Ethanol Upregulates NMDA Receptor Subunit Gene Expression in Human Embryonic Stem Cell-Derived Cortical Neurons.PLOS ONE. 2015. 10(8).
18. Tanaka, Yoshiaki*; Hysolli, Eriona; Su, Juan; Xiang, Yangfei; Kim, Kun-Yong; Zhong, Mei; Li, Yumei; Heydari, Kartoosh; Euskirchen, Ghia; Snyder, Michael P.; Pan, Xinghua; Weissman, Sherman Morton; Park, In-Hyun^#.Transcriptome Signature and Regulation in Human Somatic Cell Reprogramming.STEM CELL REPORTS. 2015. 4(6):1125-1139.