Gene Editing Center (GEC) seeks to perform innovative research and develop new technologies with intellectual property rights and translational potentials. GEC also builds up an international team of research and professionals familiar to the policies and regulations of the biomedical industry. According to national strategicplan in gene editing field, GEC will focus on development of gene editing tools, delivery and preparation of gene editing vectors, gene editing therapy, rapid detection of virus and CRISPR mechanism. In the future, GEC aims to become a comprehensive and international innovation center in the gene editing field.
RESEARCH AREARESEARCH AREA
1
Development of high precision editing tool
High-efficiency and high-accuracy single base editing system
Optimization of gene modification system with improved efficiency
2
Gene editing therapy
Gene editing therapy for thalassemia
Gene editing therapy for juvenile macular malnutrition
3
Preparation and delivery of gene editing vector
Delivery of novel gene modification tools by non-viral vectors
Exploring chemical modification of new gene modification tools
4
Rapid detection of virus
Design and synthesis of crRNA library
Screening of suitable Cas13 homologs and optimization
Optimization of nucleic acid amplification system
Optimization of crRNA
Optimization of nucleic acid cleavage system
5
Exploration, improvement and application of CRISPR system
Mitochondria and chloroplasts are organelles that include their own genomes, which encode key genes for ATP production and carbon dioxide fixation, respectively. Mutations in mitochondrial DNA can cause diverse genetic disorders and are also linked to ageing and age-related diseases, including cancer. Targeted editing of organellar DNA should be useful for studying organellar genes and developing novel therapeutics, but it has been hindered by lack of efficient tools in living cells. Recently, C
Fusing apolipoprotein B mRNA-editing enzyme, catalytic polypeptide-like cytidine deaminase with catalytically impaired Cas proteins (e.g., nCas9 or dCas9) provides a novel gene-editing technology, base editing, that grants targeted base substitutions with high efficiency. However, genome-wide and transcriptome-wide off-target mutations are observed in base editing, which raises safety concerns regarding therapeutic applications. Previously, we developed a new base editing system, the transformer
Obesity induced by high-fat diet (HFD) is a multi-factorial disease including genetic, physiological, behavioral, and environmental components.Drosophilahas emerged as an effective metabolic disease model. Cytidine 5-triphosphate synthase (CTPS) is an important enzyme for the de novo synthesis of CTP, governing the cellular level of CTP and the rate of phospholipid synthesis. CTPS is known to form filamentous structures called cytoophidia, which are found in bacteria, archaea, and eukaryotes. Ou
Chemicals or drugs can accumulate within biomolecular condensates formed through phase separation in cells. Here, we use super-resolution imaging to search for chemicals that induce phase transition within chromatin at the microscale. This microscopic screening approach reveals that adriamycin (doxorubicin) - a widely used anticancer drug that is known to interact with chromatin - specifically induces visible local condensation and global conformational change of chromatin in cancer and primary
Ruptured ectopic pregnancy (REP), a pregnancy complication caused by aberrant implantation, deep invasion, and overgrowth of embryos in fallopian tubes, could lead to rupture of fallopian tubes and accounts for 4%-10% of pregnancy-related deaths. The lack of ectopic pregnancy phenotypes in rodents hampers our understanding of its pathological mechanisms. Here, we employed cell culture and organoid models to investigate the crosstalk between human trophoblast development and intravillous vascular
